Detailed information of TA system TA170076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1185226..1186022
• Replicon: chromosome
• Accession: NZ_CP060159
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: XFGV230_RS05105
• Protein ID: WP_004088112.1
• Coordinates: 1185226..1185837 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: XFGV230_RS05110
• Protein ID: WP_004088114.1
• Coordinates: 1185834..1186022 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFGV230_RS05060	1180681..1181568	-	888	WP_012382597.1	YdaU family protein	-
XFGV230_RS05065	1181565..1182353	-	789	WP_011097897.1	Bro-N domain-containing protein	-
XFGV230_RS05070	1182350..1182982	-	633	WP_004088094.1	Bro-N domain-containing protein	-
XFGV230_RS05075	1182979..1183263	-	285	WP_004088096.1	hypothetical protein	-
XFGV230_RS05080	1183260..1183508	-	249	WP_011097898.1	hypothetical protein	-
XFGV230_RS05085	1183505..1183693	-	189	WP_004088102.1	hypothetical protein	-
XFGV230_RS05090	1183721..1183912	-	192	WP_004088104.1	hypothetical protein	-
XFGV230_RS05095	1184171..1184416	-	246	WP_004088108.1	hypothetical protein	-
XFGV230_RS05100	1184501..1185175	+	675	WP_004088110.1	helix-turn-helix transcriptional regulator	-
XFGV230_RS05105	1185226..1185837	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
XFGV230_RS05110	1185834..1186022	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
XFGV230_RS05115	1186414..1186950	+	537	WP_038233020.1	hypothetical protein	-
XFGV230_RS05120	1186947..1187360	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
XFGV230_RS05125	1187357..1187758	+	402	WP_004088054.1	hypothetical protein	-
XFGV230_RS05130	1187755..1187904	+	150	WP_012382602.1	hypothetical protein	-
XFGV230_RS05135	1187990..1188268	+	279	WP_004088050.1	hypothetical protein	-
XFGV230_RS05140	1188291..1188482	+	192	WP_012382603.1	hypothetical protein	-
XFGV230_RS05145	1188503..1188847	+	345	WP_004088346.1	hypothetical protein	-
XFGV230_RS05150	1188887..1189708	+	822	WP_004088345.1	DUF2303 family protein	-
XFGV230_RS05155	1189724..1190650	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1155062..1195772	40710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T170076 WP_004088112.1 NZ_CP060159:c1185837-1185226 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T170076 NZ_CP077079:c877602-877315 [Pseudomonas asgharzadehiana]
ATGACTCAATTTAACGATTTCGACATAGCCGAACACCTGACGAGCCCTCAGGACATTGCGGGGTATCTGGAAGCTTCTTT
CGAGGAGGACGCCGGTGACGGGCTGCTGATTCGTTCAGCGCTCAACAACATCGCCCGCGCCCAAGGCATGACCCAAATCG
CCCGCGACGCAGGTCTCGGACGGGAAAGTCTATACAAAGCCCTCTCCAGCACTGGCAATCCTGAGTTCGCGACTATCATG
AAGGTCATGAAGGCGTTAGGCCTGAGACTTCGCGCCACCGCTATCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT170076 WP_004088114.1 NZ_CP060159:c1186022-1185834 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT170076 NZ_CP077079:c877908-877606 [Pseudomonas asgharzadehiana]
ATGATTCACTTCAAGAGGTCTGATGAGTTTCAGGAATGGCTTTATTCGTTGCGCAGTCAACCCGCCCATGGACGCGTACT
AACAAGATTGGATAACGCAAGAATGGGTAATTTTGGAGACTGCGAAAACGTCGGAAATGGCATATCCGAAATGCGCATCC
ACTATGGCCCTGGATACCGTGTGTACTTCAAGCGTGTAGGTGAGGTGGTTTACCTATTGCTGACAGGTGGCGATAAATCA
ACGCAAAAACGCGACATTGAGCGCGCTAAAGCAATTGCCGATGAATTTGAGAGAAAGGAGTGA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551