Detailed information of TA system TA170074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1158020..1158625
• Replicon: chromosome
• Accession: NZ_CP060159
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: XFGV230_RS04875
• Protein ID: WP_004089252.1
• Coordinates: 1158020..1158316 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: XFGV230_RS04880
• Protein ID: WP_004089254.1
• Coordinates: 1158320..1158625 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFGV230_RS04850	1153187..1154191	-	1005	WP_128723193.1	peptidase	-
XFGV230_RS04855	1154351..1154548	-	198	WP_012382581.1	hypothetical protein	-
XFGV230_RS04860	1155062..1156225	-	1164	WP_038232954.1	phage tail protein	-
XFGV230_RS04865	1156229..1156789	-	561	WP_014607744.1	DUF2612 domain-containing protein	-
XFGV230_RS04870	1156786..1157921	-	1136	Protein_941	baseplate J/gp47 family protein	-
XFGV230_RS04875	1158020..1158316	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFGV230_RS04880	1158320..1158625	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
XFGV230_RS04885	1158636..1158989	-	354	WP_011097986.1	hypothetical protein	-
XFGV230_RS04890	1158986..1159627	-	642	WP_004090764.1	hypothetical protein	-
XFGV230_RS04895	1159624..1160454	-	831	WP_004090766.1	hypothetical protein	-
XFGV230_RS04900	1160451..1160768	-	318	WP_011097873.1	hypothetical protein	-
XFGV230_RS04905	1160768..1161538	-	771	WP_004090769.1	hypothetical protein	-
XFGV230_RS04910	1161649..1161876	+	228	WP_004091377.1	hypothetical protein	-
XFGV230_RS04915	1161860..1162126	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1155062..1195772	40710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T170074 WP_004089252.1 NZ_CP060159:1158020-1158316 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T170074 NZ_CP077078:c5855624-5855259 [Pseudomonas azerbaijanoriens]
ATGAAATGGGACGTCGAATACACGGATGAATTTGAGGCCTGGTGGGATCGCCTGAACGAGGCCGAACAGGACTCGGTTCA
GGCCACGGTGATGTTGCTGGGCGAGGATGGCCCACACTTGGGTTTTCCACACACCAGCGATATCAAGGGTTCACGGCATG
GGAACCTACGTGAGTTAAGGGTACAGCACGCTGGCAGGCCGTACCGTGTGCTGTACGCGTTTGATCCACGGCGCAGCGCG
ATCCTGCTGATTGGCGGCGATAAAACCGGCCAGGAGCGTTGGTATGCGGAAAACGTGCCGCTGGCCGAGCGTCTCTACGA
CGAGCACTTGGAGACATTGATGAAAGAGGGTTATGACAATGGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT170074 WP_004089254.1 NZ_CP060159:1158320-1158625 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT170074 NZ_CP077078:c5855266-5854961 [Pseudomonas azerbaijanoriens]
ATGGCTAAAAAATTTGCTGATTTGGTGGCACGTCGCTCTCCAGAGTCTCAAGCGCGTGTCGAGGCCTTGCATCAGAAGCT
ACGAGCTCAAATGCCGTTGCACGAGTTGCGTCAGGCGCAAGCGTTGAGTCAGGACACACTGGCCAGGACACTGCATGTCA
ACCAGGCGGCGATCTCCAAGATGGAGCGGCGAACGGACATGTACATCAGTACTTTGCGCAACTACATCCGCGCCATGGGT
GGCGAGCTGGAAATCATCGCCACTTTCCCCGAGGGCCAGGTAAAGATCGAGAACTTTGCCAACTGA

Structures

Toxin

• AlphaFold DB: A0A060HBK9