Detailed information of TA system TA170068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 984068..985305
• Replicon: chromosome
• Accession: NZ_CP060156
• Organism: Streptococcus pneumoniae strain PZ900700608

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: H6X75_RS05010
• Protein ID: WP_000405365.1
• Coordinates: 984544..985305 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: H6X75_RS05005
• Protein ID: WP_000579599.1
• Coordinates: 984068..984544 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H6X75_RS04985 (H6X75_04980)	979141..979995	+	855	WP_000900017.1	ADP-ribosylglycohydrolase family protein	-
H6X75_RS04990 (H6X75_04985)	980107..980991	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
H6X75_RS04995 (H6X75_04990)	981154..982893	+	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
H6X75_RS05000 (H6X75_04995)	983168..983623	+	456	WP_000500465.1	hypothetical protein	-
H6X75_RS05005 (H6X75_05000)	984068..984544	+	477	WP_000579599.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
H6X75_RS05010 (H6X75_05005)	984544..985305	+	762	WP_000405365.1	type II toxin-antitoxin system toxin PezT	Toxin
H6X75_RS05015 (H6X75_05010)	985333..986109	+	777	WP_001821017.1	hypothetical protein	-
H6X75_RS05020	986112..986489	+	378	WP_241409328.1	ATP-binding protein	-
H6X75_RS05025 (H6X75_05015)	987308..987667	+	360	WP_000003745.1	SAG1252 family conjugative relaxosome accessory protein	-
H6X75_RS05030 (H6X75_05020)	987677..988042	+	366	WP_000263051.1	plasmid mobilization relaxosome protein MobC	-
H6X75_RS05035 (H6X75_05025)	988029..989894	+	1866	WP_000261794.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	963668..996841	33173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T170068 WP_000405365.1 NZ_CP060156:984544-985305 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T170068 NZ_CP077076:c5330939-5330757 [Pseudomonas fakonensis]
ATGCGCAGCAGGGAAGTGATTCAGCTCATCGAAGCCAATGGCTGGTACGAGGTGGATGTCAAAGGGAGCCATCACCAATT
CAGGCATCCGTTCAAGAAAGGTCGGGTCACGGTGCCTCACCCCAAGTGCGACCTGCCCCGGGGTACGGTGCACAGCATCC
TGAAACAAGCAGGCCTGAAATGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT170068 WP_000579599.1 NZ_CP060156:984068-984544 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT170068 NZ_CP077076:c5330700-5330293 [Pseudomonas fakonensis]
ATGAAATTTCCAGTTGTACTGCACAAGGATGCGGGCTCTGACTACGGCGTGACCGTCCCCGACGTGCCTGGCTGCTTCTC
TGCCGGAGCCACCGTCTCCCAGGCGCTGGAGAATGTGCAGGAAGCCCTGGCCCTGCACTTCGAGGGTTTGGTGGCCGACG
GTGAGCCGCTGCCCCAGGCCGAGGAAGTGGACGTGCATGTGCTCAACCCTGATTACGCCGGTGGTGTGTGGGCGGTGGTG
GATTTTGATGTGACGCCCTACCTGGGCAAGGCGGTGCGGTTCAATGCCACGTTGCCGGAGAACCTGTTGCAGCGCATCGA
CGAGAAGGTCAAGCGTGACCATCGCTACGCCTCGCGTTCGGGCTTTCTGGCATCGGCTGCGTTGCGCGAGCTGGCCGTGG
CTCATTGA