Detailed information of TA system TA170067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2839352..2839829
• Replicon: chromosome
• Accession: NZ_CP060141
• Organism: Staphylococcus aureus strain 2868B2

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: H5U44_RS14230
• Protein ID: WP_000052076.1
• Coordinates: 2839641..2839829 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: H5U44_RS14225
• Protein ID: WP_001059690.1
• Coordinates: 2839352..2839612 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U44_RS14190 (2834744)	2834744..2835460	-	717	WP_031589686.1	type I 3-dehydroquinate dehydratase	-
H5U44_RS14195 (2835608)	2835608..2836030	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
H5U44_RS14200 (2836429)	2836429..2836923	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
H5U44_RS14205 (2837079)	2837079..2837696	+	618	WP_000255553.1	LysE/ArgO family amino acid transporter	-
H5U44_RS14210 (2837769)	2837769..2838350	-	582	WP_001140697.1	histidine phosphatase family protein	-
H5U44_RS14215 (2838575)	2838575..2838811	+	237	WP_000368687.1	hypothetical protein	-
H5U44_RS14220 (2838808)	2838808..2839011	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
H5U44_RS14225 (2839352)	2839352..2839612	+	261	WP_001059690.1	hypothetical protein	Antitoxin
H5U44_RS14230 (2839641)	2839641..2839829	+	189	WP_000052076.1	hypothetical protein	Toxin
H5U44_RS14235 (2840017)	2840017..2840586	+	570	WP_001106652.1	lipoprotein N-acylation protein LnsA	-
H5U44_RS14240 (2840674)	2840674..2840958	+	285	WP_031590186.1	hypothetical protein	-
H5U44_RS14245 (2841020)	2841020..2841238	+	219	WP_000005887.1	hypothetical protein	-
H5U44_RS14250 (2841734)	2841734..2841934	-	201	WP_001059082.1	cold-shock protein	-
H5U44_RS14255 (2842291)	2842291..2842977	-	687	WP_001641381.1	thermonuclease family protein	-
H5U44_RS14260 (2843318)	2843318..2843794	-	477	WP_000824477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T170067 WP_000052076.1 NZ_CP060141:2839641-2839829 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T170067 NZ_CP077076:c2210447-2210172 [Pseudomonas fakonensis]
ATGGAGTTGAAGTGGACCAGCAAGGGGCTGTCCGACCTGGCACGCCTGCATGAATTTCTAGCTGCCGTGAACCCATCTGC
TGCAGCGCGCACCGTTCAACAGCTCGCAGCAGCACCTAACGTTCTGCTGACCAACCCTCGGGTAGGAGAGCGGCTGGAAG
AATTCGAACCACGCGAAGTGCGACGGATTCTGGTTGGTCATTACGAAATGCGATATGAGATCACGCCTTCTTGCATCTAC
CTGCTGCGCCTGTGGCATACCCGCGAAGAACGCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT170067 WP_001059690.1 NZ_CP060141:2839352-2839612 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT170067 NZ_CP077076:c2210719-2210447 [Pseudomonas fakonensis]
ATGACTATCGCCGCCAAAACCCGTTCAGTTACGGCCCATGTACCCGTGGAATTGGCCGAGCGCATCGATGAAATCGCCAC
CCGGCTCGAGCGCTCAAAGAACTGGATTGTCAAACAGGCGCTGTCTGACTGGCTGGCTCAGGAAGATGAACGAAACCGCC
TGACCCTTGAAGCACTGGCAGATGTCGATGCCGGCAGAGTGATCGATCATCAGTCTGTTCAGGCTTGGGCTGAAAGCCTG
GGCACGGACTCCCCCCTGCCGGTACCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56