Detailed information of TA system TA170057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_XRE(antitoxin)
• Location: 1625992..1626789
• Replicon: chromosome
• Accession: NZ_CP060141
• Organism: Staphylococcus aureus strain 2868B2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H5U44_RS08060
• Protein ID: WP_000525014.1
• Coordinates: 1625992..1626453 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0C2LD36
• Locus tag: H5U44_RS08065
• Protein ID: WP_001260487.1
• Coordinates: 1626466..1626789 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U44_RS08025 (1621393)	1621393..1622430	-	1038	WP_000857172.1	site-specific integrase	-
H5U44_RS08030 (1622592)	1622592..1622885	-	294	WP_224397069.1	hypothetical protein	-
H5U44_RS08035 (1622931)	1622931..1623296	-	366	WP_000440839.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H5U44_RS08040 (1623636)	1623636..1623803	-	168	WP_001166555.1	hypothetical protein	-
H5U44_RS08045 (1623902)	1623902..1624255	-	354	WP_000528938.1	hypothetical protein	-
H5U44_RS08050 (1624576)	1624576..1625460	-	885	WP_001025527.1	hypothetical protein	-
H5U44_RS08055 (1625529)	1625529..1625975	-	447	WP_001226135.1	hypothetical protein	-
H5U44_RS08060 (1625992)	1625992..1626453	-	462	WP_000525014.1	hypothetical protein	Toxin
H5U44_RS08065 (1626466)	1626466..1626789	-	324	WP_001260487.1	helix-turn-helix transcriptional regulator	Antitoxin
H5U44_RS08070 (1626953)	1626953..1627201	+	249	WP_000272856.1	helix-turn-helix transcriptional regulator	-
H5U44_RS08075 (1627214)	1627214..1627657	+	444	WP_000435352.1	hypothetical protein	-
H5U44_RS08080 (1627672)	1627672..1627821	+	150	WP_000771849.1	hypothetical protein	-
H5U44_RS08085 (1627862)	1627862..1628074	-	213	WP_000362645.1	hypothetical protein	-
H5U44_RS08090 (1628143)	1628143..1628340	+	198	WP_031838117.1	hypothetical protein	-
H5U44_RS08095 (1628327)	1628327..1628549	-	223	Protein_1583	hypothetical protein	-
H5U44_RS08100 (1628616)	1628616..1628759	+	144	WP_000939498.1	hypothetical protein	-
H5U44_RS08105 (1628910)	1628910..1629230	+	321	WP_001120202.1	DUF771 domain-containing protein	-
H5U44_RS08110 (1629227)	1629227..1629388	+	162	WP_001285953.1	DUF1270 family protein	-
H5U44_RS08115 (1629487)	1629487..1629747	+	261	WP_000291082.1	DUF1108 family protein	-
H5U44_RS08120 (1629755)	1629755..1629991	+	237	WP_000802314.1	hypothetical protein	-
H5U44_RS08125 (1630014)	1630014..1630625	+	612	WP_001105358.1	MBL fold metallo-hydrolase	-
H5U44_RS08130 (1630638)	1630638..1630961	+	324	WP_000171560.1	hypothetical protein	-
H5U44_RS08135 (1630972)	1630972..1631730	+	759	WP_001003951.1	DUF1351 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL / hlb	1604208..1664585	60377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18104.44 Da
• Isoelectric Point: 4.6915

>T170057 WP_000525014.1 NZ_CP060141:c1626453-1625992 [Staphylococcus aureus]
MGLYEETLIQHDYIEVREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVYRYKEI

Nucleotide

• Length: 462 bp

>T170057 NZ_CP077074:3022245-3022817 [Pseudomonas sessilinigenes]
ATGAACGATCAACTCCTCGGTGGTCGCCTCGAATTCCTGCGCCAGGCGGAGCGCCTCAAGGACGTACTGCGCAGTGCCCA
CACCTCCAGCGGTCGCCAGGAAAGCACCGCCGAGCACAGCTGGCGCCTGTGCCTGATGGCCTTGCTGCTGGAAGACCAGC
TGGGCGAGCTGGACCTTTTGCAAGTGCTCAAGCTGTGCATCGTCCACGACCTGGGCGAAGCCCTTCATGGCGACATCCCG
GCGGTGCAGCAGGCGGCCCACCCGGACAAGGGCCAGCAGGAACGCGAAGACCTGCAGACCCTCGCCAGTTGCCTCGATGC
CCCGGCCCGCCAGCGCCTGCTGGCCCTGTGGGACGAATACGAGAATGCCAGCTCGGCCGAGGCCCAGGCGGTCAAGGCCC
TGGATAAGCTGGAGACCCTGGTGCAGCACAACCAGGGGCGTAACCCGGCGGATTTCGATTACCGCTTCAACCTCGACTAC
GGCCGCCGCTACACTAGCGCCACGCCGCTGTTCCGAGCCCTGCGCGAGCAGATCGACCACGACACGCGGCGCCACATCGA
CGCGGCCGGCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12431.09 Da
• Isoelectric Point: 9.3811

>AT170057 WP_001260487.1 NZ_CP060141:c1626789-1626466 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 324 bp

>AT170057 NZ_CP077074:3021595-3022152 [Pseudomonas sessilinigenes]
ATGTCCGCATCCTTGTTCACTTCCCCGCACCTGGGCCTGGCCCTGCGCCGCTGGCGCCTGCTGCACCGGGTCAAGCAACA
GCACGCCGCCGAGCTGTTCGGGGTGGCGCAGTCGAGCATTTCCCGCTGGGAAAACGGCCAGCAAGCCATGGAACCCAGCG
AGCGCGCCAAGCTGGAGCAACTGCTGGCGGCCAACCTCAGCGCCGCCGCCGACCAGGCCCTGGCCCGGTTGGTGAACGAC
AGCCCGCGGGCGGTGCACCTGGTCTGCGACCTGACCCACCGCCTGCTGGCCTGCTCGCCTACCCGTGCCGGGGAGTTCGG
CGTGCCCTTGAGCGAGTTGCTGGGGCAATCCTTGTGGGGCTTTTGCACCGAGGAAATCGTGCGCAAGGAGAGGGCCCTCG
ACGATCTTGGCTGGCGCGAAGTGCTAGCGCCCCCGGCGCTGGAGTTCTTCAGCGGGCACAACGATTCGGCCATCGTGCCC
ATCGCGGCCAGCCAGTGCCGCTGGACCCGCCTGAACCTGTCCGACGGCAACGCGGTGCGGCTGGTGGAAACCCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0C2LD36