Detailed information of TA system TA170056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1559676..1560205
• Replicon: chromosome
• Accession: NZ_CP060141
• Organism: Staphylococcus aureus strain 2868B2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H5U44_RS07720
• Protein ID: WP_000621175.1
• Coordinates: 1559843..1560205 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: H5U44_RS07715
• Protein ID: WP_000948331.1
• Coordinates: 1559676..1559846 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U44_RS07685 (1554712)	1554712..1555272	+	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-
H5U44_RS07690 (1555481)	1555481..1555960	+	480	WP_031590204.1	hypothetical protein	-
H5U44_RS07695 (1555953)	1555953..1557536	+	1584	WP_001294626.1	PH domain-containing protein	-
H5U44_RS07700 (1557523)	1557523..1558014	+	492	WP_072353916.1	PH domain-containing protein	-
H5U44_RS07705 (1558018)	1558018..1558377	+	360	WP_000581197.1	holo-ACP synthase	-
H5U44_RS07710 (1558443)	1558443..1559591	+	1149	WP_001281154.1	alanine racemase	-
H5U44_RS07715 (1559676)	1559676..1559846	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
H5U44_RS07720 (1559843)	1559843..1560205	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H5U44_RS07725 (1560555)	1560555..1561556	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
H5U44_RS07730 (1561675)	1561675..1562001	+	327	WP_001052491.1	anti-sigma factor antagonist	-
H5U44_RS07735 (1562003)	1562003..1562482	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
H5U44_RS07740 (1562457)	1562457..1563227	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T170056 WP_000621175.1 NZ_CP060141:1559843-1560205 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T170056 NZ_CP077074:c2834441-2834259 [Pseudomonas sessilinigenes]
GTGCAAAGCAGACAGCTAATCAAGGAGCTTGAAGCCGCAGGATGGATACTGGACCGGATAACCGGAAGCCACCATCTGTT
CAAGCATCCTTACAGACCCAACACCGTTCCGGTGCCTCACCCGAAGAAGGACCTGCCACGGGGAACGGTCAGGGCCATCC
GAAAACTGGCCGGACTGATATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT170056 WP_000948331.1 NZ_CP060141:1559676-1559846 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT170056 NZ_CP077074:c2834223-2833822 [Pseudomonas sessilinigenes]
ATGCAATATCCCATCTGCATCGAATGGGGCGACGAACACACCGCCACCGGCATCCAGATTCCCGACATCCCCGGCGCAGT
CACCGCCGGCGACACCTTCGAGAGCGCCTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGCG
CCGGACAAGCCATTCCCCTGCCCTCACCCACCCACAAACACCGCGTCAACCCCGATTTCGCGGGCATGGGCTGGGGCATG
CTGGACATCGACATCACGCCGTACCTGGGCAAGACCGAGAAGGTCAACGTGACGCTGCCAGGTTATGTGATTCAGCAGAT
CGACCGCTTTGTCCGTGAGCACAACATCAAGAGCCGGTCGTCCTTCCTGGCCGATGCCGCGCTGGAAAAGCTCGGGCGCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1