Detailed information of TA system TA170044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2721128..2721711
• Replicon: chromosome
• Accession: NZ_CP060140
• Organism: Nodularia sphaerocarpa UHCC 0038

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BDGGKGIB_RS10990
• Protein ID: WP_239731946.1
• Coordinates: 2721128..2721475 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BDGGKGIB_RS10995
• Protein ID: WP_239731948.1
• Coordinates: 2721469..2721711 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BDGGKGIB_RS10975 (BDGGKGIB_02224)	2716583..2716783	+	201	WP_239731941.1	hypothetical protein	-
BDGGKGIB_RS10980 (BDGGKGIB_02225)	2716823..2719147	-	2325	WP_239731943.1	hypothetical protein	-
BDGGKGIB_RS10985 (BDGGKGIB_02227)	2720278..2721126	+	849	WP_239731945.1	lysophospholipid acyltransferase family protein	-
BDGGKGIB_RS10990 (BDGGKGIB_02228)	2721128..2721475	-	348	WP_239731946.1	endoribonuclease MazF	Toxin
BDGGKGIB_RS10995 (BDGGKGIB_02229)	2721469..2721711	-	243	WP_239731948.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BDGGKGIB_RS11000 (BDGGKGIB_02230)	2721836..2723200	-	1365	WP_239731950.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
BDGGKGIB_RS11005 (BDGGKGIB_02231)	2723389..2725071	-	1683	WP_239731951.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-111)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12845.92 Da
• Isoelectric Point: 7.2752

>T170044 WP_239731946.1 NZ_CP060140:c2721475-2721128 [Nodularia sphaerocarpa UHCC 0038]
MVTPANSYIPSRGDIVYLDFDPTKGHEQRGHRPAFVISPQVYNEKTSLALLMPITKQQKGYPFEVILPSGLKTHGVILAD
QMKCLDWKARGVQFVESVPPEVTEEVQAKISPLLL

Nucleotide

• Length: 348 bp

>T170044 NZ_CP077073:c347933-347742 [Pseudomonas muyukensis]
ATGAACAGCCGTGAAATGATTTCGGTGATCGAGGCCGATGGTTGGTACCTGGTGCGTTGCAAAGGCAGCCACCATCACTT
CAAGCACCCCGACAAACCTGGGCTGGTAACCATCCCGCACCCGAAGAAGGACCTGCTGCCCGCCACCGCGGCCAGCATCC
TGCGCCAGGCGCGGATCGTCTACGCCAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8745.10 Da
• Isoelectric Point: 6.4613

>AT170044 WP_239731948.1 NZ_CP060140:c2721711-2721469 [Nodularia sphaerocarpa UHCC 0038]
MVANIAKWGNSLAIRIPQNLAKEIHLTEGSEVNLSVVDGNLVIKLKKPKRYSLEALIEQITPDNLHAEVDSGVAVGKEAW

Nucleotide

• Length: 243 bp

>AT170044 NZ_CP077073:c347724-347311 [Pseudomonas muyukensis]
ATGTTGTTTCCCATCGCCATCCTTCCCGGTGACGACCAGTACGCTTGGGGCGTGGAAGTACCGGACATCCCTGGCTGCTT
TTCGGCGGGTGAAGACCTCGACAACGCCATCGCCATGGCCCGCGAGGCGATCGAGGGCCATCTCGAGCTGCTTGCCCAGG
ACCAGCAGGAGATCCCCAAGGCCAGCAAGGTCACCGCGCATGCGGCCAATCCGGCCTACGCCGGTTGCACCTGGGCGTTG
ATCGATATCGATATCACCCGCTACCTGGGCAAGGCCGAGAAGCTCAACATCACCCTGCCGGCCTACCTGCTCACCCGCAT
CGACAGCTACGTGCAGAACCACCCCGAACACAAGAGCCGCTCGGGCTTTCTGGCCGAGGCGGCGCTGAAGATCCTCCAGG
GCGAGTGCAAGTAG