Detailed information of TA system TA170029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 4244366..4245885
• Replicon: chromosome
• Accession: NZ_CP060122
• Organism: Sphingobium yanoikuyae strain A3

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: H3V42_RS20280
• Protein ID: WP_185703670.1
• Coordinates: 4244971..4245885 (+)
• Length: 305 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: H3V42_RS20275
• Protein ID: WP_185703668.1
• Coordinates: 4244366..4244974 (+)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V42_RS20265	4240493..4240792	-	300	WP_185703664.1	DUF736 domain-containing protein	-
H3V42_RS20270	4241458..4243527	-	2070	WP_185703666.1	ParB/RepB/Spo0J family partition protein	-
H3V42_RS20275	4244366..4244974	+	609	WP_185703668.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
H3V42_RS20280	4244971..4245885	+	915	WP_185703670.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H3V42_RS20285	4245999..4246781	+	783	WP_185703672.1	phage Gp37/Gp68 family protein	-
H3V42_RS20290	4246815..4248068	+	1254	WP_185703674.1	three-Cys-motif partner protein TcmP	-
H3V42_RS20295	4248138..4250204	-	2067	WP_185703676.1	UvrD-helicase domain-containing protein	-
H3V42_RS20300	4250209..4250586	-	378	WP_185703678.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-270)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-52)

Sequences

Toxin

Protein

• Length: 305 a.a.
• Molecular weight: 33411.50 Da
• Isoelectric Point: 5.4656

>T170029 WP_185703670.1 NZ_CP060122:4244971-4245885 [Sphingobium yanoikuyae]
MSRPPKNMAISVRDRLTARARERRENAQLLMTRYVIERLLFRLSLSPHRERFVLKGAMLFSLWAAAPYRATGDLDLLGMG
ENAPEQLAAVFQEILGVAVDDDGIVFRPETLRAAAARAEDEYAGVRIDFVAELAGARLPMHVDIGYGDAISPGAIEIEYP
SLLGQPTAHLRAYPPETVVAEKFQAMVALGMLNTRLKDFYDIWAIAGSFAFDGTVLSRAIQATFDRRQTPLPVETPPALT
PAFADEKQGQWTAFLRRTEIALAPEPFAAIQAQIASLVMPPVLALGQGTALASDWPIGGPWKER

Nucleotide

• Length: 915 bp

>T170029 NZ_CP077061:c8848-8447 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22393.89 Da
• Isoelectric Point: 9.8244

>AT170029 WP_185703668.1 NZ_CP060122:4244366-4244974 [Sphingobium yanoikuyae]
MEPTTHRQRALEVVRHRGIARGRDFDAAGVTRATLQRLRDEGVLQQVGRGLYKLADADLDSATSLAEAVRIQPRGIICLL
SALQFHELTTQTPHAVWMMLGQKDWAPVNPSVALKIVRASGEALTAGVETHLIDGVPVPITVPAKTVADCFKHRNKIGID
VAVEALRDFMKSRPRPDLNAIYRYAQLDRVQSVIRPYLEAMT

Nucleotide

• Length: 609 bp

>AT170029 NZ_CP077061:c9018-8848 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA