Detailed information of TA system TA170023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4410907..4411721
• Replicon: chromosome
• Accession: NZ_CP060121
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: FFHJCLDM_RS21245
• Protein ID: WP_001054376.1
• Coordinates: 4411464..4411721 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: FFHJCLDM_RS21240
• Protein ID: WP_001309181.1
• Coordinates: 4410907..4411452 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFHJCLDM_RS21215	4406598..4407911	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
FFHJCLDM_RS21220	4407923..4408201	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
FFHJCLDM_RS21225	4408198..4409319	-	1122	WP_000010829.1	M42 family metallopeptidase	-
FFHJCLDM_RS21230	4409718..4409936	-	219	Protein_4132	hypothetical protein	-
FFHJCLDM_RS21235	4410105..4410851	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
FFHJCLDM_RS21240	4410907..4411452	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
FFHJCLDM_RS21245	4411464..4411721	-	258	WP_001054376.1	hypothetical protein	Toxin
FFHJCLDM_RS21250	4412098..4412343	-	246	Protein_4136	GNAT family N-acetyltransferase	-
FFHJCLDM_RS21255	4412713..4413699	+	987	Protein_4137	hypothetical protein	-
FFHJCLDM_RS21260	4414282..4415262	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
FFHJCLDM_RS21265	4415327..4416433	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T170023 WP_001054376.1 NZ_CP060121:c4411721-4411464 [Escherichia coli BL21]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T170023 NZ_CP076863:c4809205-4808828 [Klebsiella pneumoniae]
ATGAAAACATTACCCGACACACACGAACGGGAGGCGTCACGCTGCTCGTCTCCTGTGACAATCTGGCAGACACTCCTGGC
CCGGCTGCTGGGCCAGCATTACAGCCTCACGCTGAATGACACGCCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAGTACGCGCTGGTACGTACCGACCAGCCGGGATTC
GGCACCTGTGCCCATTCGCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGTTGACCCGCAG
CAACTACAGAACGGTGAACGACATTATCCGGGGTAAAGATGCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT170023 WP_001309181.1 NZ_CP060121:c4411452-4410907 [Escherichia coli BL21]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT170023 NZ_CP076863:c4809629-4809252 [Klebsiella pneumoniae]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAGGGTAACCGGTTGCATTACCTTGCAGATCGCGCAGGTATCAGAGGTCAGT
TCAGCAACGCGGATGCGTACCATCTGGACCGAGCCTTTCCGCTGCTGCTGAAACAACTGGAGTTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
GGGTTACGTTTATATGGCTGTTTATCCGACACCGGCAGCTCCCGCAACAACCGCATAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8