Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1432514..1433152 Replicon chromosome
Accession NZ_CP060121
Organism Escherichia coli BL21

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FFHJCLDM_RS07055 Protein ID WP_000813794.1
Coordinates 1432514..1432690 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FFHJCLDM_RS07060 Protein ID WP_001270286.1
Coordinates 1432736..1433152 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFHJCLDM_RS07035 1428133..1429347 - 1215 WP_001326689.1 BenE family transporter YdcO -
FFHJCLDM_RS07040 1429400..1429936 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FFHJCLDM_RS07045 1430009..1431970 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FFHJCLDM_RS07050 1432062..1432292 - 231 WP_000494244.1 YncJ family protein -
FFHJCLDM_RS07055 1432514..1432690 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FFHJCLDM_RS07060 1432736..1433152 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FFHJCLDM_RS07065 1433231..1434637 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FFHJCLDM_RS07070 1434882..1436027 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FFHJCLDM_RS07075 1436045..1437058 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FFHJCLDM_RS07080 1437059..1438000 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T170008 WP_000813794.1 NZ_CP060121:1432514-1432690 [Escherichia coli BL21]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T170008 NZ_CP076856:c67926-67615 [Shewanella decolorationis]
ATGCCGGTAACTTATCATTTAACGCCCGATGCACAATCTGATCTGATAGGTATTCACCGCTTTACGTTAGCCCAATGGGG
AACGACTCAGTCGAAGACCTATTTATCAGGACTTAGACAAACGATTCAACTGCTGGCTGAAACACCCACCCTTGGGAAAA
ATAGACCCGAGGTACGCATGAATGTGTTTAGCTTCCCTTATTCAAGTCATGTCATCTATTACATCCAACATGAGCATCAA
TTCGTTGTATTTGGGATCTTACATAAAAGTATGGTTCCACTTGCACATCTCGCGGAGCGGGAAATTATTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT170008 WP_001270286.1 NZ_CP060121:1432736-1433152 [Escherichia coli BL21]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT170008 NZ_CP076856:c68186-67926 [Shewanella decolorationis]
ATGAACACTCTTTCAGCCAATGAAGCAAAAATTCACTTTGGAGATTTGTTGCTCAAAGCTCAACAAGCTCCAATCCAAAT
TAATAAAAATGGCAAGCCTGTGGCCGTTGTTATTTCAGCTGATGCATACCAAAGTATCGAGACACTAAAATTACATTTGC
TTCAATCCAAAGCGGTGAAAGCCATAACTGACATCAAAATGGGCAATTTGGTTGATGGTAATACCTTTTTTGATGAGCTG
GCCGCAGGGCAGTATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References