Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 449136..449754 Replicon chromosome
Accession NZ_CP060121
Organism Escherichia coli BL21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FFHJCLDM_RS02120 Protein ID WP_001291435.1
Coordinates 449136..449354 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FFHJCLDM_RS02125 Protein ID WP_000344800.1
Coordinates 449380..449754 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFHJCLDM_RS02085 444425..444997 + 573 WP_000779826.1 lipoprotein -
FFHJCLDM_RS02090 445028..445339 - 312 WP_000409911.1 MGMT family protein -
FFHJCLDM_RS02100 445718..446071 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FFHJCLDM_RS02105 446113..447663 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FFHJCLDM_RS02110 447827..448297 - 471 WP_000136192.1 YlaC family protein -
FFHJCLDM_RS02115 448413..448964 - 552 WP_000102564.1 maltose O-acetyltransferase -
FFHJCLDM_RS02120 449136..449354 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FFHJCLDM_RS02125 449380..449754 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FFHJCLDM_RS02130 450300..453449 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FFHJCLDM_RS02135 453472..454665 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T170001 WP_001291435.1 NZ_CP060121:c449354-449136 [Escherichia coli BL21]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T170001 NZ_CP076853:c781589-781299 [Rhizobium sp. WYJ-E13]
ATGAAATACCGCACCACGTTTGAAGCAGATCGGGATATTATCGAGATTTACGTTCTCGGCGCCGAGCAGTTCGGCGTTCC
GCAGTCCGAGCGATACGTTAGTGAGTTGTTCGGCACATTCGAATTGCTTGCCGATAATCCGCAAATGGCGCGGGAGCGCC
AGGAACTGACCCCGCCCATGCGGCTTCATCCTGATCAGGCACATCTGATTGCCTACACTATCCGGGAGGATGACATCCTG
ATCGTTCGCGTCCTGCACAGTCGGCAGGATTGGCAAAGTCTTTTCACCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT170001 WP_000344800.1 NZ_CP060121:c449754-449380 [Escherichia coli BL21]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT170001 NZ_CP076853:c781834-781586 [Rhizobium sp. WYJ-E13]
ATGGCAACCATGAACGTTTCCCTGCCGGACCCGATGAAGGAATGGGTGGAAGCCCAGACAAGGACGGGGCGTTACAGCAA
CGCAAGCGACTATGTCCGTGATCTGATCCGGCGCGATCAGGAGCGGTCGGACAAGCTGGCAGAACTGCAACGGCTTCTTG
CCGAAGGTTTGGAGAGTGGCGTCAGTGACCGCTCAAAGGAAGATATTCTCCGGGCTGCGCGTGAACGATTGGCCGCCGGC
CAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References