Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1447733..1448351 Replicon chromosome
Accession NZ_CP060117
Organism Shigella sonnei strain UKMCC_1015

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HYI41_RS06980 Protein ID WP_001291435.1
Coordinates 1447733..1447951 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HYI41_RS06985 Protein ID WP_000344800.1
Coordinates 1447977..1448351 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI41_RS06955 (1442983) 1442983..1443336 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HYI41_RS06960 (1443378) 1443378..1444926 - 1549 Protein_1346 cyclic-guanylate-specific phosphodiesterase PdeB -
HYI41_RS06965 (1445887) 1445887..1445888 - 2 WP_086558215.1 IS3-like element IS2 family transposase -
HYI41_RS06970 (1446425) 1446425..1446895 - 471 WP_000136192.1 YlaC family protein -
HYI41_RS06975 (1447011) 1447011..1447562 - 552 WP_000102564.1 maltose O-acetyltransferase -
HYI41_RS06980 (1447733) 1447733..1447951 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HYI41_RS06985 (1447977) 1447977..1448351 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HYI41_RS06990 (1448897) 1448897..1452046 - 3150 WP_001132464.1 efflux RND transporter permease AcrB -
HYI41_RS06995 (1452069) 1452069..1453262 - 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169980 WP_001291435.1 NZ_CP060117:c1447951-1447733 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T169980 NZ_CP060117:c1447951-1447733 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT169980 WP_000344800.1 NZ_CP060117:c1448351-1447977 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT169980 NZ_CP060117:c1448351-1447977 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References