Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 709626..710123 Replicon chromosome
Accession NZ_CP060117
Organism Shigella sonnei strain UKMCC_1015

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HYI41_RS03370 Protein ID WP_001173343.1
Coordinates 709950..710123 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HYI41_RS03365 Protein ID WP_000398619.1
Coordinates 709626..709922 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI41_RS03345 (706357) 706357..707076 - 720 WP_000611285.1 two-component system response regulator DcuR -
HYI41_RS03350 (707073) 707073..708704 - 1632 WP_001216495.1 sensor histidine kinase -
HYI41_RS03355 (708885) 708885..709115 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
HYI41_RS03360 (709127) 709127..709399 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HYI41_RS03365 (709626) 709626..709922 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HYI41_RS03370 (709950) 709950..710123 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HYI41_RS03375 (710242) 710242..711759 - 1518 WP_001295086.1 lysine--tRNA ligase -
HYI41_RS03380 (711996) 711996..713453 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
HYI41_RS03385 (713512) 713512..713769 - 258 Protein_647 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T169977 WP_001173343.1 NZ_CP060117:709950-710123 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T169977 NZ_CP076838:1999393-2000727 [Leclercia pneumoniae]
ATGCCTAAGCTCGTGACGTGGATGAACAATGAGCGAGTTGGTGAGCTAACAAAGCTCCCTAACGGCGCGCATACCTTTAA
ATATGATCGTGAGTGGCTCTATAACCGCCACGCCAGGCCCATCTCGCTGTCGCTTCCCTTGCAGACCGGCACGCTCACCT
CTGATGCGGTCTTCAATTTCTTTGACAACCTGTTGCCGGATAGCGCTGTCGTGCGGGATCGCATTGTTAAACGTTATCAG
GCGCGCTCAAAACAGCCCTTCGATCTGCTGGCGGAAGTGGGCCGCGACAGCGTCGGTGCCGTGACCCTGCTGCCCCCGGA
TGCGATCATCTCTGCCCCGATCATGCGCTGGGAGACGCTGGATGAACCCCGGCTCGAAAAGGTGCTCTCTGCTTATCAGG
CCGATATTCCGCTTGGCATGATTAAAGACGAGAACGATTTTCGTATTTCGGTGGCGGGCGCCCAGGAAAAGACGGCGCTG
TTACAGATTAACGACGCGTGGTGCATTCCGAAGGGGATAACCCCCACCAACCATATTATTAAGCTGCCCATCGGGGAGAT
CAGACAGCCCAATGCCACCCTGGATCTTCGCGACAGCGTGGATAACGAATATTTCTGTCTGGCCTTTGCCAGGGCGCTGG
GGCTGGCGGTTCCTGACGCCACCATTATTACCGCCGGGCAGATTCGGGCGCTAGCCGTGGAACGTTTTGACCGCCGCTGG
AACGCCGATCGCACTATGTTGCTGCGATTGCCGCAGGAGGATATGTGTCAGGCCTTTGGTTTACCCTCTTCGTTGAAGTA
TGAATCCGACGGCGGGCCCGGTATTTTGCCGATTATGTCGTTTTTGCTGGGATCGAGTGAAGCCCTGCAGGATCGCTATC
ACTTTATGCAGTTCCAGCTCTTTCAGTGGCTTCTGGCCGCGACGGACGGGCACGCCAAAAACTTCTCGGTGTTCATCCAG
CCTGGGGGCAGTTACCGTCTAACCCCGTTTTACGACATTCTTTCAGCTTATCCGCTGCTCGGCGGGGCGGGTCTGCACAT
CAGCGACCTGAAGCTGGCGATGGGGCTCAAGGCTTCCAGAGGGCGCAAGAACGCAATCGATCGTATTTATCCGCGTCATT
TTTTTGCGCTGGCAAAAGAAGTGGGGTTTGCACAAAGCGAGATGGAGAGGATCATGCAAACCTTCGCCCAACAAGTTCCT
GATGCGCTGCGTCGCGTGAACGATGCGCTTCCGGCTGGTTTCTCCAGCAGGGTAGCTAAAACCATTACCGCCGGGGTGCT
GCGACTGCATCAGCGTCTGGTAAATGGATTATCCCTTACAGGGTATAAATACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT169977 WP_000398619.1 NZ_CP060117:709626-709922 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT169977 NZ_CP076838:1999124-1999393 [Leclercia pneumoniae]
ATGAAACCACCCATGATCTACAGCCCCGTGCAGCTGGCAAATTACCTGAAATTAACGCGTCAGAAGAATAACTGGACCCA
GAGTGAACTGGCCCGAAAGATTGGTATCAAGCAAGCCACGGTGTCGAATTTTGAAAACAACCCCGACAACATCACCCTGA
CCACTTTCTTCAAGCTATTACAGGCGCTGGAGTTAACGATGGTGCTATATGATAAGGGTGAAACGGTCGAGGATAGCACC
TCTATTCAGCATCAGGATGTAGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References