169974

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5328393..5328969 Replicon chromosome
Accession NZ_CP060111
Organism Klebsiella michiganensis strain AKKL-001

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag H5403_RS25025 Protein ID WP_042933975.1
Coordinates 5328682..5328969 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag H5403_RS25020 Protein ID WP_025108476.1
Coordinates 5328393..5328695 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5403_RS25000 5323809..5323973 - 165 WP_004098259.1 DUF1127 domain-containing protein -
H5403_RS25005 5324151..5325575 + 1425 WP_004098258.1 PLP-dependent aminotransferase family protein -
H5403_RS25010 5326021..5326932 + 912 WP_185219634.1 acetamidase/formamidase family protein -
H5403_RS25015 5326929..5328272 + 1344 WP_004128506.1 APC family permease -
H5403_RS25020 5328393..5328695 - 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
H5403_RS25025 5328682..5328969 - 288 WP_042933975.1 BrnT family toxin Toxin
H5403_RS25030 5329221..5329664 - 444 WP_064375701.1 FosA family fosfomycin resistance glutathione transferase -
H5403_RS25035 5329658..5330566 - 909 WP_014837311.1 LysR family transcriptional regulator -
H5403_RS25040 5330654..5331436 + 783 WP_122117630.1 NAD(P)H-dependent oxidoreductase -
H5403_RS25045 5331584..5332168 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
H5403_RS25050 5332186..5332986 + 801 WP_064358752.1 winged helix-turn-helix domain-containing protein -
H5403_RS25055 5332983..5333501 + 519 WP_004098240.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T169974 WP_042933975.1 NZ_CP060111:c5328969-5328682 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T169974 NZ_CP076838:707405-707821 [Leclercia pneumoniae]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCGTGGCGCTCACAGTGGATGTCTCTTTTACTCCACGGGTTGGTTGCGGC
CGCCATTCTGTTAATGCCATGGCCGCTGAGCTATACCCCGCTCTGGCTGCTACTTCTCTCGTTGGTGGTCTTTGACAGCG
TTAGAAGCCAGCGGCGGATCAACGCCCGTCAGGGTGAAATCAAACTGTTTATGGATTCCCGCCTGCGCTGGCAGGGGAAA
GAGTGGGAGATCGTCGGCTCGCCGTGGATGATTAACGCCGGCATGATGCTGCGTCTGCGTGCAAGCGAAGAGCAGCGCTG
CCACCATTTATGGGTATCGGCGGATAGCATGGATGAAGGGGAGTGGCGAGACCTGCGTCGGATGATTGCTCAGCAACAGC
CGACGCAAGGACGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT169974 WP_025108476.1 NZ_CP060111:c5328695-5328393 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT169974 NZ_CP076838:707158-707424 [Leclercia pneumoniae]
ATGGATATTAATAATAAAGCCCGCATTCACTGGGCATGCCGTCGCGGTATGCGTGAACTCGACATCTCCATCATGCCGTT
CTTCGAACATGAATACGACAGCCTGAGCGATGAAGACAAACAGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCAGATT
TATTCAACTGGCTGATGAATCATGGCGAACCAGCCGACAGCGAGCTGCAACGGATGGTGCTTTTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References