Detailed information of TA system TA169963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2060553..2061143
• Replicon: chromosome
• Accession: NZ_CP060111
• Organism: Klebsiella michiganensis strain AKKL-001

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: H5403_RS09740
• Protein ID: WP_185220000.1
• Coordinates: 2060811..2061143 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: H5403_RS09735
• Protein ID: WP_004852307.1
• Coordinates: 2060553..2060810 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5403_RS09710	2055810..2056418	-	609	WP_057174013.1	glutathione S-transferase N-terminal domain-containing protein	-
H5403_RS09715	2056595..2057521	+	927	WP_014838892.1	LysR family transcriptional regulator	-
H5403_RS09720	2057559..2058557	-	999	WP_185219999.1	aldo/keto reductase	-
H5403_RS09725	2058658..2059572	+	915	WP_025106070.1	LysR family transcriptional regulator	-
H5403_RS09730	2059691..2059960	-	270	WP_185220221.1	hypothetical protein	-
H5403_RS09735	2060553..2060810	+	258	WP_004852307.1	antitoxin	Antitoxin
H5403_RS09740	2060811..2061143	+	333	WP_185220000.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H5403_RS09750	2061466..2062923	+	1458	WP_025106072.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
H5403_RS09760	2063886..2065340	-	1455	WP_014229909.1	AMP nucleosidase	-
H5403_RS09765	2065473..2065730	-	258	WP_004852295.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11753.57 Da
• Isoelectric Point: 10.1863

>T169963 WP_185220000.1 NZ_CP060111:2060811-2061143 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFNVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T169963 NZ_CP076823:851430-851750 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCAGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCAGAGAACTTTGACGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGAGTA
A

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT169963 WP_004852307.1 NZ_CP060111:2060553-2060810 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT169963 NZ_CP076823:851218-851427 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8