Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107831..108378 Replicon chromosome
Accession NZ_CP060111
Organism Klebsiella michiganensis strain AKKL-001

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag H5403_RS00515 Protein ID WP_014227299.1
Coordinates 107831..108139 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag H5403_RS00520 Protein ID WP_014227298.1
Coordinates 108142..108378 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5403_RS00490 103735..104046 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
H5403_RS00495 104341..105282 + 942 WP_025107585.1 LacI family transcriptional regulator -
H5403_RS00500 105306..105599 - 294 WP_064357598.1 hypothetical protein -
H5403_RS00505 105809..106774 + 966 WP_064357600.1 Rpn family recombination-promoting nuclease/putative transposase -
H5403_RS00510 106778..107680 - 903 WP_014227300.1 EamA family transporter -
H5403_RS00515 107831..108139 - 309 WP_014227299.1 CcdB family protein Toxin
H5403_RS00520 108142..108378 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H5403_RS00525 108484..109917 - 1434 WP_048262922.1 PTS N-acetylmuramic acid transporter subunit IIBC -
H5403_RS00530 109942..110844 - 903 WP_185219739.1 N-acetylmuramic acid 6-phosphate etherase -
H5403_RS00535 111005..112171 - 1167 WP_014227296.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T169958 WP_014227299.1 NZ_CP060111:c108139-107831 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T169958 NZ_CP076819:6630-6917 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT169958 WP_014227298.1 NZ_CP060111:c108378-108142 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT169958 NZ_CP076819:6904-7218 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References