Detailed information of TA system TA169945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5441547..5442199
• Replicon: chromosome
• Accession: NZ_CP060086
• Organism: Pseudomonas aeruginosa strain JNQH-PA57

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5409_RS25175
• Protein ID: WP_003134109.1
• Coordinates: 5441547..5441957 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5409_RS25180
• Protein ID: WP_170869361.1
• Coordinates: 5441957..5442199 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5409_RS25135	5437829..5438242	-	414	WP_153570846.1	hypothetical protein	-
H5409_RS25140	5438253..5438393	+	141	WP_153549538.1	hypothetical protein	-
H5409_RS25145	5438550..5439278	+	729	WP_023094843.1	TIGR03761 family integrating conjugative element protein	-
H5409_RS25150	5439284..5439817	+	534	WP_003134105.1	DUF3158 family protein	-
H5409_RS25155	5439831..5440319	+	489	WP_003134107.1	single-stranded DNA-binding protein	-
H5409_RS25160	5440423..5440617	+	195	WP_031632157.1	hypothetical protein	-
H5409_RS25165	5440869..5441036	+	168	WP_128729268.1	CrpP family protein	-
H5409_RS25170	5441337..5441531	-	195	WP_023094845.1	hypothetical protein	-
H5409_RS25175	5441547..5441957	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H5409_RS25180	5441957..5442199	-	243	WP_170869361.1	antitoxin	Antitoxin
H5409_RS25185	5442505..5444424	+	1920	WP_003134111.1	type I DNA topoisomerase	-
H5409_RS25190	5444432..5445184	+	753	Protein_4982	hypothetical protein	-
H5409_RS25195	5445301..5445906	-	606	WP_023094848.1	LysE family translocator	-
H5409_RS25200	5446028..5446897	+	870	WP_023094849.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	5424238..5542003	117765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (11-37)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T169945 WP_003134109.1 NZ_CP060086:c5441957-5441547 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T169945 NZ_CP076806:c8025-7669 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8909.94 Da
• Isoelectric Point: 4.4230

>AT169945 WP_170869361.1 NZ_CP060086:c5442199-5441957 [Pseudomonas aeruginosa]
MRCVVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 243 bp

>AT169945 NZ_CP076806:c7676-7374 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA