Detailed information of TA system TA169944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4996702..4997310
• Replicon: chromosome
• Accession: NZ_CP060086
• Organism: Pseudomonas aeruginosa strain JNQH-PA57

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: H5409_RS23140
• Protein ID: WP_121382286.1
• Coordinates: 4996702..4997049 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: H5409_RS23145
• Protein ID: WP_003114155.1
• Coordinates: 4997059..4997310 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5409_RS23110	4992061..4992333	+	273	WP_003134390.1	cysteine-rich CWC family protein	-
H5409_RS23115	4992333..4993025	+	693	WP_003140963.1	16S rRNA pseudouridine(516) synthase	-
H5409_RS23120	4993161..4994204	+	1044	WP_003134392.1	L,D-transpeptidase	-
H5409_RS23125	4994284..4995021	+	738	WP_003140965.1	murein L,D-transpeptidase catalytic domain family protein	-
H5409_RS23130	4995473..4996375	+	903	WP_003085447.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
H5409_RS23140	4996702..4997049	-	348	WP_121382286.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5409_RS23145	4997059..4997310	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5409_RS23150	4997524..4998507	-	984	WP_121382283.1	tyrosine-type recombinase/integrase	-
H5409_RS23155	4998507..4999799	-	1293	WP_121382281.1	hypothetical protein	-
H5409_RS23160	5000029..5001312	-	1284	WP_003140504.1	zonular occludens toxin family protein	-
H5409_RS23165	5001316..5001672	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4996702..5009996	13294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12982.80 Da
• Isoelectric Point: 4.4212

>T169944 WP_121382286.1 NZ_CP060086:c4997049-4996702 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQLVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T169944 NZ_CP076806:2953-3240 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT169944 WP_003114155.1 NZ_CP060086:c4997310-4997059 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT169944 NZ_CP076806:3227-3541 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355