Detailed information of TA system TA169936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3412541..3413178
• Replicon: chromosome
• Accession: NZ_CP060085
• Organism: Bacillus velezensis strain HAB-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: H5405_RS16660
• Protein ID: WP_003156187.1
• Coordinates: 3412541..3412891 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: H5405_RS16665
• Protein ID: WP_003156188.1
• Coordinates: 3412897..3413178 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5405_RS16620	3407581..3408183	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
H5405_RS16625	3408183..3408971	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
H5405_RS16630	3408937..3409419	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
H5405_RS16635	3409416..3409745	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
H5405_RS16640	3409809..3410816	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
H5405_RS16645	3410828..3411229	-	402	WP_003156178.1	anti-sigma regulatory factor	-
H5405_RS16650	3411232..3411597	-	366	WP_007609584.1	RsbT antagonist protein RsbS	-
H5405_RS16655	3411602..3412423	-	822	WP_003156182.1	STAS domain-containing protein	-
H5405_RS16660	3412541..3412891	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
H5405_RS16665	3412897..3413178	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
H5405_RS16670	3413298..3414467	-	1170	WP_007609581.1	alanine racemase	-
H5405_RS16675	3414584..3415591	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
H5405_RS16680	3415756..3416121	-	366	WP_007609580.1	holo-ACP synthase	-
H5405_RS16685	3416214..3416813	+	600	WP_007609579.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T169936 WP_003156187.1 NZ_CP060085:c3412891-3412541 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T169936 NZ_CP076742:1452165-1452347 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT169936 WP_003156188.1 NZ_CP060085:c3413178-3412897 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT169936 NZ_CP076742:1452412-1452816 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATCGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAACGTAACTTTACCTAGCCGTTTAATTCGCAAGAT
TGATGATAATGTAGGTAAAGATAAAAGATTTAAAACTCGTTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5