Detailed information of TA system TA169935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2670166..2671083
• Replicon: chromosome
• Accession: NZ_CP060085
• Organism: Bacillus velezensis strain HAB-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: H5405_RS12765
• Protein ID: WP_007407256.1
• Coordinates: 2670166..2670912 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: H5405_RS12770
• Protein ID: WP_003154807.1
• Coordinates: 2670913..2671083 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5405_RS12745	2665558..2666508	-	951	WP_007610844.1	ring-cleaving dioxygenase	-
H5405_RS12750	2666831..2668147	+	1317	WP_007610842.1	amino acid permease	-
H5405_RS12755	2668433..2669050	+	618	WP_003154804.1	DUF47 domain-containing protein	-
H5405_RS12760	2669063..2670061	+	999	WP_007610840.1	inorganic phosphate transporter	-
H5405_RS12765	2670166..2670912	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
H5405_RS12770	2670913..2671083	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
H5405_RS12775	2671340..2672218	-	879	WP_007610836.1	N-acetylmuramoyl-L-alanine amidase	-
H5405_RS12780	2672232..2672495	-	264	WP_003154813.1	phage holin	-
H5405_RS12785	2672509..2672772	-	264	WP_003154815.1	hemolysin XhlA family protein	-
H5405_RS12790	2672824..2673585	-	762	WP_029325867.1	hypothetical protein	-
H5405_RS12795	2673642..2673839	-	198	WP_007610833.1	XkdX family protein	-
H5405_RS12800	2673844..2674215	-	372	WP_007610831.1	hypothetical protein	-
H5405_RS12805	2674228..2675859	-	1632	WP_007610826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T169935 WP_007407256.1 NZ_CP060085:2670166-2670912 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T169935 NZ_CP076742:706243-706632 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT169935 WP_003154807.1 NZ_CP060085:2670913-2671083 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT169935 NZ_CP076742:705979-706236 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14