Detailed information of TA system TA169925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2764870..2765508
• Replicon: chromosome
• Accession: NZ_CP060083
• Organism: Escherichia coli strain G1/2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2X7GI71
• Locus tag: H4P46_RS13545
• Protein ID: WP_032231106.1
• Coordinates: 2765332..2765508 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H4P46_RS13540
• Protein ID: WP_001270286.1
• Coordinates: 2764870..2765286 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P46_RS13520	2760022..2760963	-	942	WP_001251313.1	ABC transporter permease	-
H4P46_RS13525	2760964..2761977	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H4P46_RS13530	2761995..2763140	-	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
H4P46_RS13535	2763385..2764791	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H4P46_RS13540	2764870..2765286	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H4P46_RS13545	2765332..2765508	-	177	WP_032231106.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H4P46_RS13550	2765730..2765960	+	231	WP_000494244.1	YncJ family protein	-
H4P46_RS13555	2766052..2768013	-	1962	WP_001491066.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H4P46_RS13560	2768086..2768622	-	537	WP_000429151.1	DNA-binding transcriptional regulator SutR	-
H4P46_RS13565	2768675..2769886	+	1212	WP_071528280.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6680.72 Da
• Isoelectric Point: 10.9223

>T169925 WP_032231106.1 NZ_CP060083:c2765508-2765332 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T169925 NZ_CP076728:2302082-2302819 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT169925 WP_001270286.1 NZ_CP060083:c2765286-2764870 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT169925 NZ_CP076728:2302932-2303057 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2X7GI71