Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2601592..2602118 Replicon chromosome
Accession NZ_CP060083
Organism Escherichia coli strain G1/2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H4P46_RS12740 Protein ID WP_000323025.1
Coordinates 2601831..2602118 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H4P46_RS12735 Protein ID WP_000534858.1
Coordinates 2601592..2601831 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P46_RS12715 2596954..2597310 + 357 WP_001310834.1 class I SAM-dependent methyltransferase -
H4P46_RS12720 2598977..2600290 + 1314 WP_047644851.1 ISNCY family transposase -
H4P46_RS12725 2600727..2601059 - 333 WP_001301033.1 protein FlxA -
H4P46_RS12730 2601262..2601567 - 306 WP_001326990.1 hypothetical protein -
H4P46_RS12735 2601592..2601831 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H4P46_RS12740 2601831..2602118 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H4P46_RS12745 2602190..2602345 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H4P46_RS12750 2602562..2602813 + 252 WP_000980994.1 hypothetical protein -
H4P46_RS12755 2602880..2603158 + 279 WP_012304870.1 hypothetical protein -
H4P46_RS12760 2603160..2604209 + 1050 WP_001265198.1 DUF968 domain-containing protein -
H4P46_RS12765 2604223..2604975 + 753 WP_001047135.1 antitermination protein -
H4P46_RS12770 2605253..2605342 - 90 WP_120795389.1 hypothetical protein -
H4P46_RS12775 2605397..2605609 - 213 WP_000087756.1 cold shock-like protein CspF -
H4P46_RS12780 2605910..2606125 + 216 WP_000066484.1 cold shock-like protein CspB -
H4P46_RS12785 2606879..2607094 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2491600..2655310 163710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T169923 WP_000323025.1 NZ_CP060083:2601831-2602118 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T169923 NZ_CP076726:3268029-3268163 [Xanthomonas arboricola pv. juglandis]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT169923 WP_000534858.1 NZ_CP060083:2601592-2601831 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT169923 NZ_CP076726:3267794-3267946 [Xanthomonas arboricola pv. juglandis]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References