Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1043759..1044342 Replicon chromosome
Accession NZ_CP060083
Organism Escherichia coli strain G1/2

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag H4P46_RS04950 Protein ID WP_000254738.1
Coordinates 1044007..1044342 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H4P46_RS04945 Protein ID WP_000581937.1
Coordinates 1043759..1044007 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P46_RS04935 1040098..1041399 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
H4P46_RS04940 1041447..1043681 + 2235 WP_000226815.1 GTP pyrophosphokinase -
H4P46_RS04945 1043759..1044007 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H4P46_RS04950 1044007..1044342 + 336 WP_000254738.1 endoribonuclease MazF Toxin
H4P46_RS04955 1044413..1045204 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H4P46_RS04960 1045432..1047069 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
H4P46_RS04965 1047157..1048455 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T169911 WP_000254738.1 NZ_CP060083:1044007-1044342 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T169911 NZ_CP076723:c4337722-4337393 [Geomonas oryzisoli]
ATGAAACGGGGAGATGTCGTCCTGGTGACCACTGCAGGTGATTATGGCAAACCACGACCTGCTCTTGTGGTCCAAACCGA
CTACTTTTCTGAGCACCCAAGCGTAACGCTCTGTCTCCTGACATCCTTTATGTTGGATGCCCCGCTCTATCGTTACAAGC
TCGCACCGACGGCAGAGAATGGGCTGTCAGCCCCCTCTTTTGTTCAGGTGGACAAGCTAATGACCGTGCCCCGCCAAAAA
GTAGGTGATGTCATAGGGAACTTGTCTGACAAACAGATGTCCGAGATCACGAGACTATTGGCATTGTGGATAGGCATCGC
GGACAGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT169911 WP_000581937.1 NZ_CP060083:1043759-1044007 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT169911 NZ_CP076723:c4337925-4337719 [Geomonas oryzisoli]
ATGCAGCCGACAAGAGATAGAGTGCAAAATTACAGGGCAAAACTTCGTGAAGCTGGATTGAAGCCGGTTCAGATATGGGT
TCCCGATCCAGCGACCCCTGGTTTTGAGGCCGAATGCCTCAGACAATCGCGGCTCGCGCTTCAAGACCCGCAGAATCTGC
AGGACTTGGAAGACATGGCTGAGGTGGCCGACTGGGGTGAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References