Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 928158..928812 Replicon chromosome
Accession NZ_CP060083
Organism Escherichia coli strain G1/2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H4P46_RS04460 Protein ID WP_000244777.1
Coordinates 928405..928812 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H4P46_RS04455 Protein ID WP_000354046.1
Coordinates 928158..928424 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P46_RS04430 923327..924070 + 744 WP_000951964.1 SDR family oxidoreductase -
H4P46_RS04435 924127..925560 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H4P46_RS04440 925605..925916 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H4P46_RS04445 926080..926739 + 660 WP_000250274.1 hemolysin III family protein -
H4P46_RS04450 926935..927915 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H4P46_RS04455 928158..928424 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H4P46_RS04460 928405..928812 + 408 WP_000244777.1 toxin CptA Toxin
H4P46_RS04465 928852..929373 - 522 WP_001055874.1 flavodoxin FldB -
H4P46_RS04470 929485..930381 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H4P46_RS04475 930406..931116 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H4P46_RS04480 931122..932855 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T169910 WP_000244777.1 NZ_CP060083:928405-928812 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T169910 NZ_CP076723:c4319452-4319171 [Geomonas oryzisoli]
GTGATAAAAACGTTCGCAGACAAAGACACGAAGCAGCTCTTCCTGACAGGTAAGGCTAAGCGGCTACCTCCCGATTTGTT
CGGAAGGGCAAGACGGCGCCTGGAATACATAGACCTTGCCTCAGTCCTTGAAGACCTTAAAGTGCCTCCGAGTAACAGGT
TGCATCCTTTGCATGGCAGCCGATACGGCCAGCATGCCATCTCGATCAATGACCAGTGGCGGATTTGTTTTGTTTGGCAC
AACGGCGATGCGTATGAAGTGGAGATAACGGATTATCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT169910 WP_000354046.1 NZ_CP060083:928158-928424 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT169910 NZ_CP076723:c4319162-4318848 [Geomonas oryzisoli]
ATGTTTAAAAGTGACCGAGACAAAAGAGAAATTCCGCCAACCCATCCGGGAGCAATGCTGCGCGAAGACTTCATGCCGGA
TTATGGGTTGACTGCAGCAACGCTGGCGGGGACGTTGGGAGTGTCGCGGCAAACTATTAACGAACTGCTCCGCGAACGCA
GGGCGCTGAGCCCGGGGATGGCCCTGAGACTTTCCCGCCTTTTCGGTAACACACCTGAGTTCTGGCTGAACGCACAGCGC
GCTGTTGATCTATGGCAGGCGGAAAAGGATTTGGCAAAAGAACTCGCCAGGATCCGGTCGCTTCAAGCCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References