Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3896730..3897409 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag H4P47_RS19140 Protein ID WP_000854680.1
Coordinates 3897068..3897409 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag H4P47_RS19135 Protein ID WP_000070396.1
Coordinates 3896730..3897047 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS19090 3892168..3892989 + 822 WP_047400937.1 DUF945 domain-containing protein -
H4P47_RS19095 3893206..3893907 + 702 WP_000189411.1 WYL domain-containing protein -
H4P47_RS19100 3893948..3894184 + 237 WP_001144031.1 hypothetical protein -
H4P47_RS19105 3894184..3894627 + 444 WP_000649865.1 hypothetical protein -
H4P47_RS19110 3894650..3895117 + 468 WP_001385283.1 hypothetical protein -
H4P47_RS19115 3895194..3895433 + 240 WP_000194654.1 DUF905 domain-containing protein -
H4P47_RS19120 3895531..3895989 + 459 WP_001548150.1 antirestriction protein -
H4P47_RS19125 3896005..3896481 + 477 WP_000811693.1 RadC family protein -
H4P47_RS19130 3896490..3896711 + 222 WP_016246875.1 DUF987 domain-containing protein -
H4P47_RS19135 3896730..3897047 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
H4P47_RS19140 3897068..3897409 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
H4P47_RS19145 3897915..3898136 - 222 WP_159105992.1 hypothetical protein -
H4P47_RS19150 3898273..3900606 - 2334 WP_000783674.1 toprim domain-containing protein -
H4P47_RS19155 3900621..3900941 - 321 WP_000856729.1 DUF5375 domain-containing protein -
H4P47_RS19160 3901077..3901532 - 456 WP_000459315.1 hypothetical protein -
H4P47_RS19165 3901525..3901812 - 288 WP_001244665.1 hypothetical protein -
H4P47_RS19170 3901805..3902404 - 600 WP_047400944.1 host cell division inhibitor Icd-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3892168..3925457 33289


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T169902 WP_000854680.1 NZ_CP060075:3897068-3897409 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T169902 NZ_CP076712:65970-66119 [Escherichia coli O158:H23]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACGGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT169902 WP_000070396.1 NZ_CP060075:3896730-3897047 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT169902 NZ_CP076712:c65926-65865 [Escherichia coli O158:H23]
TTGAGATACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References