169901

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3759098..3759935 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H4P47_RS18440 Protein ID WP_000227784.1
Coordinates 3759393..3759935 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H4P47_RS18435 Protein ID WP_001297137.1
Coordinates 3759098..3759409 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS18410 3754118..3755065 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
H4P47_RS18415 3755087..3757078 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H4P47_RS18420 3757068..3757682 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H4P47_RS18425 3757682..3758011 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H4P47_RS18430 3758023..3758913 + 891 WP_000971336.1 heme o synthase -
H4P47_RS18435 3759098..3759409 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H4P47_RS18440 3759393..3759935 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H4P47_RS18445 3759991..3760926 - 936 WP_001297127.1 sel1 repeat family protein -
H4P47_RS18450 3761334..3762698 + 1365 WP_032314626.1 MFS transporter -
H4P47_RS18455 3762826..3763317 - 492 WP_001138904.1 nucleotide binding protein YajQ -
H4P47_RS18460 3763485..3764396 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T169901 WP_000227784.1 NZ_CP060075:3759393-3759935 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T169901 NZ_CP076712:c56816-56418 [Escherichia coli O158:H23]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT169901 WP_001297137.1 NZ_CP060075:3759098-3759409 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT169901 NZ_CP076712:c57043-56816 [Escherichia coli O158:H23]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACATAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References