Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3725020..3725638 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H4P47_RS18270 Protein ID WP_001291435.1
Coordinates 3725420..3725638 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H4P47_RS18265 Protein ID WP_000344800.1
Coordinates 3725020..3725394 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS18255 3720109..3721302 + 1194 WP_047400767.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4P47_RS18260 3721325..3724474 + 3150 WP_047400769.1 efflux RND transporter permease AcrB -
H4P47_RS18265 3725020..3725394 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H4P47_RS18270 3725420..3725638 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H4P47_RS18275 3725809..3726360 + 552 WP_000102569.1 maltose O-acetyltransferase -
H4P47_RS18280 3726476..3726946 + 471 WP_000136192.1 YlaC family protein -
H4P47_RS18285 3727110..3728660 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H4P47_RS18290 3728702..3729055 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H4P47_RS18300 3729434..3729745 + 312 WP_047400772.1 MGMT family protein -
H4P47_RS18305 3729776..3730348 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169900 WP_001291435.1 NZ_CP060075:3725420-3725638 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T169900 NZ_CP076711:c56831-56682 [Escherichia coli O158:H23]
ATGACGAAGTATGCCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAGTATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT169900 WP_000344800.1 NZ_CP060075:3725020-3725394 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT169900 NZ_CP076711:56876-56935 [Escherichia coli O158:H23]
AACGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References