Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2658690..2659328 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H4P47_RS12930 Protein ID WP_000813794.1
Coordinates 2659152..2659328 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H4P47_RS12925 Protein ID WP_001270286.1
Coordinates 2658690..2659106 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS12905 2653842..2654783 - 942 WP_001251313.1 ABC transporter permease -
H4P47_RS12910 2654784..2655797 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
H4P47_RS12915 2655815..2656960 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
H4P47_RS12920 2657205..2658611 - 1407 WP_016247173.1 PLP-dependent aminotransferase family protein -
H4P47_RS12925 2658690..2659106 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H4P47_RS12930 2659152..2659328 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H4P47_RS12935 2659550..2659780 + 231 WP_000494244.1 YncJ family protein -
H4P47_RS12940 2659872..2661833 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H4P47_RS12945 2661906..2662442 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H4P47_RS12950 2662495..2663709 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T169899 WP_000813794.1 NZ_CP060075:c2659328-2659152 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T169899 NZ_CP076711:c53768-53481 [Escherichia coli O158:H23]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT169899 WP_001270286.1 NZ_CP060075:c2659106-2658690 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT169899 NZ_CP076711:c54016-53765 [Escherichia coli O158:H23]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAGCGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References