Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2543044..2543780 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H4P47_RS12420 Protein ID WP_003026803.1
Coordinates 2543044..2543526 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H4P47_RS12425 Protein ID WP_003026799.1
Coordinates 2543514..2543780 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS12390 2539524..2539922 - 399 WP_148981364.1 hypothetical protein -
H4P47_RS12395 2540324..2540536 - 213 WP_001446195.1 cold shock-like protein CspF -
H4P47_RS12400 2540815..2541033 + 219 WP_000203924.1 cold-shock protein -
H4P47_RS12405 2541302..2541529 + 228 WP_016247217.1 hypothetical protein -
H4P47_RS12410 2542092..2542496 + 405 WP_016247216.1 H-NS histone family protein -
H4P47_RS12415 2542576..2542659 + 84 Protein_2430 arsenical resistance protein ArsH -
H4P47_RS12420 2543044..2543526 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H4P47_RS12425 2543514..2543780 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H4P47_RS12430 2544225..2544455 - 231 WP_004206660.1 hypothetical protein -
H4P47_RS12435 2544469..2544672 - 204 WP_004213596.1 hemolysin expression modulator Hha -
H4P47_RS12440 2544733..2545224 - 492 WP_016247215.1 hypothetical protein -
H4P47_RS12445 2545608..2546312 - 705 WP_016237027.1 IS6-like element IS26 family transposase -
H4P47_RS12450 2546378..2547324 + 947 Protein_2437 transposase -
H4P47_RS12455 2547448..2547897 + 450 Protein_2438 DNA starvation/stationary phase protection protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2529479..2559002 29523


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T169898 WP_003026803.1 NZ_CP060075:c2543526-2543044 [Escherichia coli]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T169898 NZ_CP076711:c8375-8070 [Escherichia coli O158:H23]
ATGCAGTTTAAAGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGACGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTAGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATTTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT169898 WP_003026799.1 NZ_CP060075:c2543780-2543514 [Escherichia coli]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT169898 NZ_CP076711:c8595-8377 [Escherichia coli O158:H23]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References