Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeV-yafW/CbtA-CbeA
Location 844172..844965 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name yeeV Uniprot ID S1EZ92
Locus tag H4P47_RS04135 Protein ID WP_000854726.1
Coordinates 844172..844549 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EBQ7
Locus tag H4P47_RS04140 Protein ID WP_001548930.1
Coordinates 844639..844965 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS04110 840230..840766 + 537 WP_016247600.1 hypothetical protein -
H4P47_RS04115 841214..842059 + 846 WP_016247599.1 hypothetical protein -
H4P47_RS04120 842551..843393 - 843 WP_001547770.1 DUF4942 domain-containing protein -
H4P47_RS04125 843478..843675 - 198 WP_000839269.1 DUF957 domain-containing protein -
H4P47_RS04130 843687..844175 - 489 WP_001547769.1 hypothetical protein -
H4P47_RS04135 844172..844549 - 378 WP_000854726.1 TA system toxin CbtA family protein Toxin
H4P47_RS04140 844639..844965 - 327 WP_001548930.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
H4P47_RS04145 844984..845205 - 222 WP_000691994.1 DUF987 domain-containing protein -
H4P47_RS04150 845214..845690 - 477 WP_000811693.1 RadC family protein -
H4P47_RS04155 845706..846164 - 459 WP_001548150.1 antirestriction protein -
H4P47_RS04160 846262..846501 - 240 WP_000194654.1 DUF905 domain-containing protein -
H4P47_RS04165 846578..847045 - 468 WP_001547765.1 hypothetical protein -
H4P47_RS04170 847068..847511 - 444 WP_001547764.1 phage transcriptional regulator -
H4P47_RS04175 847511..847747 - 237 WP_001144031.1 hypothetical protein -
H4P47_RS04180 847788..848489 - 702 WP_016239437.1 WYL domain-containing protein -
H4P47_RS04185 848706..849527 - 822 WP_000197389.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 808669..849527 40858


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-103)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.10 Da        Isoelectric Point: 7.8045

>T169885 WP_000854726.1 NZ_CP060075:c844549-844172 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Download         Length: 378 bp

>T169885 NZ_CP076709:c1428340-1428233 [Escherichia coli O158:H23]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12301.97 Da        Isoelectric Point: 7.0273

>AT169885 WP_001548930.1 NZ_CP060075:c844965-844639 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Download         Length: 327 bp

>AT169885 NZ_CP076709:1428393-1428454 [Escherichia coli O158:H23]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EZ92

Antitoxin

Source ID Structure
AlphaFold DB S1EBQ7

References