Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 311292..311923 Replicon chromosome
Accession NZ_CP060075
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H4P47_RS01400 Protein ID WP_001259388.1
Coordinates 311292..311567 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H4P47_RS01405 Protein ID WP_000593555.1
Coordinates 311564..311923 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS01385 306296..307363 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
H4P47_RS01390 307360..310095 + 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
H4P47_RS01395 310095..311219 + 1125 WP_001216257.1 ABC transporter permease -
H4P47_RS01400 311292..311567 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H4P47_RS01405 311564..311923 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H4P47_RS01410 312061..312758 + 698 Protein_278 IS1 family transposase -
H4P47_RS01415 312820..313221 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H4P47_RS01420 313227..314033 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H4P47_RS01425 314030..314794 - 765 WP_001136249.1 nickel import ATP-binding protein NikD -
H4P47_RS01430 314794..315627 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H4P47_RS01435 315624..316568 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 289320..312758 23438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T169882 WP_001259388.1 NZ_CP060075:311292-311567 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T169882 NZ_CP076708:c47638-47357 [Escherichia coli O170:H18]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTTCTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT169882 WP_000593555.1 NZ_CP060075:311564-311923 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT169882 NZ_CP076708:c47879-47628 [Escherichia coli O170:H18]
ATGAGTTATCAAATCCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
CGAGGGAGACGCAGTTGCGATCCTGAATCGAAACGAACCGGCGTTCTATTGCGTCCCCCCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCCGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAGATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References