Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3405921..3406539 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H4P48_RS16125 Protein ID WP_001291435.1
Coordinates 3406321..3406539 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H4P48_RS16120 Protein ID WP_000344800.1
Coordinates 3405921..3406295 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS16110 3401010..3402203 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H4P48_RS16115 3402226..3405375 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H4P48_RS16120 3405921..3406295 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H4P48_RS16125 3406321..3406539 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H4P48_RS16130 3406711..3407262 + 552 WP_000102564.1 maltose O-acetyltransferase -
H4P48_RS16135 3407378..3407848 + 471 WP_000136192.1 YlaC family protein -
H4P48_RS16140 3408012..3409562 + 1551 WP_021570707.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H4P48_RS16145 3409604..3409957 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H4P48_RS16155 3410336..3410647 + 312 WP_000409911.1 MGMT family protein -
H4P48_RS16160 3410678..3411250 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169877 WP_001291435.1 NZ_CP060073:3406321-3406539 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T169877 NZ_CP076706:c4049083-4048865 [Escherichia coli O170:H18]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT169877 WP_000344800.1 NZ_CP060073:3405921-3406295 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT169877 NZ_CP076706:c4049483-4049109 [Escherichia coli O170:H18]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References