Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2414682..2415320 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H4P48_RS11440 Protein ID WP_000813794.1
Coordinates 2415144..2415320 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H4P48_RS11435 Protein ID WP_001270286.1
Coordinates 2414682..2415098 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS11415 2409834..2410775 - 942 WP_001251304.1 ABC transporter permease -
H4P48_RS11420 2410776..2411789 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H4P48_RS11425 2411807..2412952 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
H4P48_RS11430 2413197..2414603 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
H4P48_RS11435 2414682..2415098 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H4P48_RS11440 2415144..2415320 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H4P48_RS11445 2415542..2415772 + 231 WP_000494244.1 YncJ family protein -
H4P48_RS11450 2415864..2417825 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H4P48_RS11455 2417898..2418434 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H4P48_RS11460 2418487..2419701 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T169876 WP_000813794.1 NZ_CP060073:c2415320-2415144 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T169876 NZ_CP076706:3840948-3841346 [Escherichia coli O170:H18]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT169876 WP_001270286.1 NZ_CP060073:c2415098-2414682 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT169876 NZ_CP076706:3840652-3840945 [Escherichia coli O170:H18]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References