Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2230894..2231420 Replicon chromosome
Accession NZ_CP060073
Organism Escherichia coli strain G4/9

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H4P48_RS10605 Protein ID WP_000323025.1
Coordinates 2231133..2231420 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H4P48_RS10600 Protein ID WP_000534858.1
Coordinates 2230894..2231133 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P48_RS10580 2227018..2228331 + 1314 WP_000589005.1 ISNCY family transposase -
H4P48_RS10590 2230029..2230361 - 333 WP_001301033.1 protein FlxA -
H4P48_RS10595 2230564..2230869 - 306 WP_001326990.1 hypothetical protein -
H4P48_RS10600 2230894..2231133 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H4P48_RS10605 2231133..2231420 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H4P48_RS10610 2231492..2231647 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H4P48_RS10615 2231864..2232115 + 252 WP_000980994.1 hypothetical protein -
H4P48_RS10620 2232182..2232460 + 279 WP_012304870.1 hypothetical protein -
H4P48_RS10625 2232462..2233511 + 1050 WP_001265198.1 DUF968 domain-containing protein -
H4P48_RS10630 2233525..2234277 + 753 WP_001047135.1 antitermination protein -
H4P48_RS10635 2234555..2234644 - 90 WP_120795389.1 hypothetical protein -
H4P48_RS10640 2234699..2234911 - 213 WP_000087756.1 cold shock-like protein CspF -
H4P48_RS10645 2235212..2235427 + 216 WP_000066484.1 cold shock-like protein CspB -
H4P48_RS10650 2236181..2236396 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2215574..2249779 34205


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T169874 WP_000323025.1 NZ_CP060073:2231133-2231420 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T169874 NZ_CP076706:c3520628-3520476 [Escherichia coli O170:H18]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT169874 WP_000534858.1 NZ_CP060073:2230894-2231133 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT169874 NZ_CP076706:3520677-3520734 [Escherichia coli O170:H18]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References