Detailed information of TA system TA169860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 91222..91823
• Replicon: plasmid unnamed1
• Accession: NZ_CP060066
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H4P49_RS23880
• Protein ID: WP_047602357.1
• Coordinates: 91443..91823 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: H4P49_RS23875
• Protein ID: WP_001190712.1
• Coordinates: 91222..91443 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS23855	87078..87839	-	762	Protein_82	restriction endonuclease subunit S	-
H4P49_RS23860	87905..88885	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
H4P49_RS23865	88986..89486	-	501	WP_187002610.1	restriction endonuclease subunit S	-
H4P49_RS23870	89483..91039	-	1557	WP_047602375.1	type I restriction-modification system subunit M	-
H4P49_RS23875	91222..91443	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H4P49_RS23880	91443..91823	+	381	WP_047602357.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H4P49_RS23885	91828..92007	+	180	WP_032162342.1	hypothetical protein	-
H4P49_RS23890	92035..93078	+	1044	WP_047602360.1	DUF968 domain-containing protein	-
H4P49_RS23895	93167..93619	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
H4P49_RS23900	93706..94899	+	1194	WP_047602363.1	terminase	-
H4P49_RS23905	94899..96413	+	1515	WP_047602367.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..148192	148192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13534.25 Da
• Isoelectric Point: 5.6343

>T169860 WP_047602357.1 NZ_CP060066:91443-91823 [Escherichia coli]
MRHISPEELIALHDANISRYGGLSGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T169860 NZ_CP076706:c236090-235386 [Escherichia coli O170:H18]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAAGCGGGAAGGGTGCGCGCGTTAGCGGT
CGAACGTTTTGACAGGCGTTGGAATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCG
GTTTACCTTCATCGGTGAAATATGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGC
GAGGCGCTGAAAGATCGCTATGATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAA
AAACTTCTCCGTATTTATTCAGGCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCC
TTGGCGGTACGGGAATACACATCAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATC
GATAAAATTTATCCGCGACATTTTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAG
TGACTTTGCCAGAATGATTCCGGCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGA
CGGCAGTTGAAACCAATGTGTTGAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT169860 WP_001190712.1 NZ_CP060066:91222-91443 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT169860 NZ_CP076706:c236356-236090 [Escherichia coli O170:H18]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAATTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6