169857

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4069197..4069773 Replicon chromosome
Accession NZ_CP060065
Organism Escherichia coli strain G5

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag H4P49_RS19640 Protein ID WP_001299718.1
Coordinates 4069486..4069773 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag H4P49_RS19635 Protein ID WP_000063159.1
Coordinates 4069197..4069499 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P49_RS19620 4065786..4067936 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
H4P49_RS19625 4067986..4068189 + 204 WP_000467859.1 YbdD/YjiX family protein -
H4P49_RS19630 4068200..4069156 + 957 WP_001299717.1 GTPase -
H4P49_RS19635 4069197..4069499 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
H4P49_RS19640 4069486..4069773 - 288 WP_001299718.1 BrnT family toxin Toxin
H4P49_RS19645 4069970..4071136 + 1167 Protein_3841 restriction endonuclease -
H4P49_RS19650 4071200..4071511 + 312 WP_047601822.1 type I restriction-modification system subunit M N-terminal domain-containing protein -
H4P49_RS19655 4071562..4072259 + 698 Protein_3843 IS1-like element IS1A family transposase -
H4P49_RS19660 4072274..4073596 + 1323 Protein_3844 SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T169857 WP_001299718.1 NZ_CP060065:c4069773-4069486 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T169857 NZ_CP076705:64479-64877 [Escherichia coli O8:H28]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACACC
GGTCAGATAAGAACAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT169857 WP_000063159.1 NZ_CP060065:c4069499-4069197 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT169857 NZ_CP076705:64252-64479 [Escherichia coli O8:H28]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References