Detailed information of TA system TA169856

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3775792..3776579
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A377E3D6
• Locus tag: H4P49_RS18265
• Protein ID: WP_001194695.1
• Coordinates: 3776202..3776579 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2H9G3E7
• Locus tag: H4P49_RS18260
• Protein ID: WP_000066236.1
• Coordinates: 3775792..3776151 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS18215	3771031..3771483	+	453	WP_047602207.1	hypothetical protein	-
H4P49_RS18220	3771480..3771932	+	453	WP_000734138.1	hypothetical protein	-
H4P49_RS18225	3771995..3772537	+	543	WP_001104018.1	DUF4339 domain-containing protein	-
H4P49_RS18230	3772597..3773049	+	453	WP_001061894.1	hypothetical protein	-
H4P49_RS18235	3773126..3773359	+	234	WP_001114682.1	DUF905 domain-containing protein	-
H4P49_RS18240	3773479..3774297	+	819	WP_001234406.1	DUF945 domain-containing protein	-
H4P49_RS18245	3774565..3775035	+	471	WP_000131762.1	antirestriction protein	-
H4P49_RS18250	3775047..3775526	+	480	WP_061355964.1	DNA repair protein RadC	-
H4P49_RS18255	3775547..3775768	+	222	WP_000691981.1	DUF987 domain-containing protein	-
H4P49_RS18260	3775792..3776151	+	360	WP_000066236.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H4P49_RS18265	3776202..3776579	+	378	WP_001194695.1	TA system toxin CbtA family protein	Toxin
H4P49_RS18270	3776576..3777067	+	492	WP_000777682.1	hypothetical protein	-
H4P49_RS18275	3777099..3777302	+	204	WP_000413747.1	DUF957 domain-containing protein	-
H4P49_RS18280	3777383..3778227	+	845	Protein_3578	DUF4942 domain-containing protein	-
H4P49_RS18290	3778569..3779300	-	732	WP_001300756.1	DNA polymerase III subunit epsilon	-
H4P49_RS18295	3779365..3779832	+	468	WP_001556414.1	ribonuclease HI	-
H4P49_RS18300	3779829..3780551	-	723	WP_001297210.1	class I SAM-dependent methyltransferase	-
H4P49_RS18305	3780585..3781340	+	756	WP_001052717.1	hydroxyacylglutathione hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14180.97 Da
• Isoelectric Point: 7.4209

>T169856 WP_001194695.1 NZ_CP060065:3776202-3776579 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Nucleotide

• Length: 378 bp

>T169856 NZ_CP076705:c56497-56348 [Escherichia coli O8:H28]
ATGACGAAATATGCCCTTATTGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13127.02 Da
• Isoelectric Point: 7.0261

>AT169856 WP_000066236.1 NZ_CP060065:3775792-3776151 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT169856 NZ_CP076705:56542-56601 [Escherichia coli O8:H28]
TAAGTACATCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A377E3D6

Antitoxin

• AlphaFold DB: A0A2H9G3E7