Detailed information of TA system TA169853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3709031..3709710
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: H4P49_RS17910
• Protein ID: WP_000854680.1
• Coordinates: 3709369..3709710 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: H4P49_RS17905
• Protein ID: WP_000070396.1
• Coordinates: 3709031..3709348 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS17860	3704469..3705290	+	822	WP_001548153.1	DUF945 domain-containing protein	-
H4P49_RS17865	3705507..3706208	+	702	WP_021553244.1	WYL domain-containing protein	-
H4P49_RS17870	3706249..3706485	+	237	WP_001144029.1	hypothetical protein	-
H4P49_RS17875	3706485..3706928	+	444	WP_021553245.1	hypothetical protein	-
H4P49_RS17880	3706951..3707418	+	468	WP_001385283.1	hypothetical protein	-
H4P49_RS17885	3707495..3707734	+	240	Protein_3500	DUF905 domain-containing protein	-
H4P49_RS17890	3707832..3708290	+	459	WP_000211838.1	antirestriction protein	-
H4P49_RS17895	3708306..3708782	+	477	WP_000811693.1	RadC family protein	-
H4P49_RS17900	3708791..3709012	+	222	WP_000691994.1	DUF987 domain-containing protein	-
H4P49_RS17905	3709031..3709348	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
H4P49_RS17910	3709369..3709710	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
H4P49_RS17915	3710479..3710952	-	474	WP_047601724.1	DUF2280 domain-containing protein	-
H4P49_RS17920	3711045..3711224	-	180	WP_047601726.1	hypothetical protein	-
H4P49_RS17925	3711221..3711883	-	663	WP_047601727.1	translation initiation factor IF-2	-
H4P49_RS17930	3711894..3712241	-	348	WP_047601729.1	hypothetical protein	-
H4P49_RS17935	3712246..3713337	-	1092	WP_047601730.1	hypothetical protein	-
H4P49_RS17940	3713343..3713510	-	168	WP_047601733.1	hypothetical protein	-
H4P49_RS17945	3713491..3713679	-	189	WP_047601735.1	hypothetical protein	-
H4P49_RS17950	3713692..3714243	-	552	WP_047601737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3654297..3738193	83896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T169853 WP_000854680.1 NZ_CP060065:3709369-3709710 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T169853 NZ_CP076704:c4318416-4318243 [Escherichia coli O8:H28]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT169853 WP_000070396.1 NZ_CP060065:3709031-3709348 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT169853 NZ_CP076704:c4318740-4318444 [Escherichia coli O8:H28]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7