Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2425511..2427099 Replicon chromosome
Accession NZ_CP060065
Organism Escherichia coli strain G5

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag H4P49_RS11820 Protein ID WP_047601200.1
Coordinates 2425511..2426833 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag H4P49_RS11825 Protein ID WP_001301023.1
Coordinates 2426833..2427099 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P49_RS11785 2420571..2420840 - 270 WP_023277128.1 hypothetical protein -
H4P49_RS11790 2420858..2421148 - 291 WP_003033731.1 hypothetical protein -
H4P49_RS11795 2421165..2422280 - 1116 WP_047602324.1 Replication-associated protein G2P -
H4P49_RS11800 2422404..2422592 + 189 WP_003033726.1 hypothetical protein -
H4P49_RS11805 2422785..2423051 + 267 WP_023277130.1 hypothetical protein -
H4P49_RS11810 2423254..2424657 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
H4P49_RS11815 2424686..2425318 - 633 WP_001567369.1 hypothetical protein -
H4P49_RS11820 2425511..2426833 - 1323 WP_047601200.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
H4P49_RS11825 2426833..2427099 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
H4P49_RS11830 2427322..2428305 - 984 Protein_2314 autotransporter outer membrane beta-barrel domain-containing protein -
H4P49_RS11835 2428362..2429059 + 698 Protein_2315 IS1-like element IS1A family transposase -
H4P49_RS11840 2429075..2429728 + 654 Protein_2316 SPASM domain-containing protein -
H4P49_RS11845 2429694..2429816 - 123 WP_120795387.1 protein YneP -
H4P49_RS11850 2430019..2431704 + 1686 WP_000628576.1 ABC transporter ATP-binding protein/permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2275098..2431704 156606
- inside IScluster/Tn - - 2423254..2429059 5805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49293.85 Da        Isoelectric Point: 8.2482

>T169850 WP_047601200.1 NZ_CP060065:c2426833-2425511 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEMGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T169850 NZ_CP076704:c4148176-4147835 [Escherichia coli O8:H28]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
TCGCCTGCTGGAACAACATTATGGCCTGACGCTAAACGACACGCCGTTCAGTGATGAAAAGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GGCTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACAGGTTTATTGAAAGCGCG
GCACGGTGAACATTGGGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT169850 WP_001301023.1 NZ_CP060065:c2427099-2426833 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT169850 NZ_CP076704:c4148538-4148197 [Escherichia coli O8:H28]
ATGAAATCTACTGATTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAATCGACTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATTTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATAAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCTCACTTATCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References