Detailed information of TA system TA169849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2308705..2309231
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H4P49_RS11165
• Protein ID: WP_000323025.1
• Coordinates: 2308944..2309231 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H4P49_RS11160
• Protein ID: WP_000534858.1
• Coordinates: 2308705..2308944 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS11135	2303917..2304267	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
H4P49_RS11140	2304264..2304689	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
H4P49_RS11145	2306090..2307403	+	1314	WP_047601174.1	ISNCY family transposase	-
H4P49_RS11150	2307840..2308172	-	333	WP_001301033.1	protein FlxA	-
H4P49_RS11155	2308375..2308680	-	306	WP_001326990.1	hypothetical protein	-
H4P49_RS11160	2308705..2308944	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H4P49_RS11165	2308944..2309231	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H4P49_RS11170	2309303..2309458	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H4P49_RS11175	2309675..2309926	+	252	WP_000980994.1	hypothetical protein	-
H4P49_RS11180	2309993..2310271	+	279	WP_012304870.1	hypothetical protein	-
H4P49_RS11185	2310273..2311322	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
H4P49_RS11190	2311336..2312088	+	753	WP_001047135.1	antitermination protein	-
H4P49_RS11195	2312366..2312455	-	90	WP_120795389.1	hypothetical protein	-
H4P49_RS11200	2312510..2312722	-	213	WP_000087756.1	cold shock-like protein CspF	-
H4P49_RS11205	2313023..2313238	+	216	WP_000066484.1	cold shock-like protein CspB	-
H4P49_RS11210	2313992..2314207	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2275098..2431704	156606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T169849 WP_000323025.1 NZ_CP060065:2308944-2309231 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T169849 NZ_CP076704:3716392-3716670 [Escherichia coli O8:H28]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT169849 WP_000534858.1 NZ_CP060065:2308705-2308944 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT169849 NZ_CP076704:3716129-3716389 [Escherichia coli O8:H28]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8