Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4821241..4821738 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H4P50_RS23740 Protein ID WP_001173343.1
Coordinates 4821241..4821414 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H4P50_RS23745 Protein ID WP_000398619.1
Coordinates 4821442..4821738 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P50_RS23730 4817911..4819368 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H4P50_RS23735 4819605..4821122 + 1518 WP_001295074.1 lysine--tRNA ligase -
H4P50_RS23740 4821241..4821414 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H4P50_RS23745 4821442..4821738 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H4P50_RS23750 4821965..4822237 - 273 WP_000405651.1 N-acetyltransferase -
H4P50_RS23755 4822249..4822479 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
H4P50_RS23760 4822660..4824291 + 1632 WP_001216477.1 sensor histidine kinase -
H4P50_RS23765 4824288..4825007 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T169832 WP_001173343.1 NZ_CP060061:c4821414-4821241 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T169832 NZ_CP076704:193489-193596 [Escherichia coli O8:H28]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT169832 WP_000398619.1 NZ_CP060061:c4821738-4821442 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT169832 NZ_CP076704:c193431-193375 [Escherichia coli O8:H28]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References