Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2142628..2143154 Replicon chromosome
Accession NZ_CP060061
Organism Escherichia coli strain G6/7

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H4P50_RS10265 Protein ID WP_000323025.1
Coordinates 2142628..2142915 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H4P50_RS10270 Protein ID WP_000534858.1
Coordinates 2142915..2143154 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P50_RS10220 2137652..2137867 - 216 WP_000839590.1 phage lysis protein EssD -
H4P50_RS10225 2138621..2138836 - 216 WP_000066484.1 cold shock-like protein CspB -
H4P50_RS10230 2139137..2139349 + 213 WP_000087756.1 cold shock-like protein CspF -
H4P50_RS10235 2139404..2139493 + 90 WP_120795389.1 hypothetical protein -
H4P50_RS10240 2139771..2140523 - 753 WP_001047135.1 antitermination protein -
H4P50_RS10245 2140537..2141586 - 1050 WP_001265198.1 DUF968 domain-containing protein -
H4P50_RS10250 2141588..2141866 - 279 WP_012304870.1 hypothetical protein -
H4P50_RS10255 2141933..2142184 - 252 WP_000980994.1 hypothetical protein -
H4P50_RS10260 2142401..2142556 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H4P50_RS10265 2142628..2142915 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H4P50_RS10270 2142915..2143154 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H4P50_RS10275 2143179..2143484 + 306 WP_001326990.1 hypothetical protein -
H4P50_RS10280 2143687..2144019 + 333 WP_001301033.1 protein FlxA -
H4P50_RS10285 2144456..2145769 - 1314 WP_047644851.1 ISNCY family transposase -
H4P50_RS10290 2147436..2147792 - 357 WP_001310834.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2089302..2253239 163937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T169815 WP_000323025.1 NZ_CP060061:c2142915-2142628 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T169815 NZ_CP076697:c3942076-3941969 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT169815 WP_000534858.1 NZ_CP060061:c2143154-2142915 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT169815 NZ_CP076697:3942133-3942190 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References