Detailed information of TA system TA169813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1979064..1979702
• Replicon: chromosome
• Accession: NZ_CP060061
• Organism: Escherichia coli strain G6/7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2X7GI71
• Locus tag: H4P50_RS09455
• Protein ID: WP_032231106.1
• Coordinates: 1979064..1979240 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H4P50_RS09460
• Protein ID: WP_001270286.1
• Coordinates: 1979286..1979702 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P50_RS09435	1974686..1975897	-	1212	WP_071528280.1	BenE family transporter YdcO	-
H4P50_RS09440	1975950..1976486	+	537	WP_000429151.1	DNA-binding transcriptional regulator SutR	-
H4P50_RS09445	1976559..1978520	+	1962	WP_001491066.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H4P50_RS09450	1978612..1978842	-	231	WP_000494244.1	YncJ family protein	-
H4P50_RS09455	1979064..1979240	+	177	WP_032231106.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H4P50_RS09460	1979286..1979702	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H4P50_RS09465	1979781..1981187	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H4P50_RS09470	1981432..1982577	+	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
H4P50_RS09475	1982595..1983608	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H4P50_RS09480	1983609..1984550	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6680.72 Da
• Isoelectric Point: 10.9223

>T169813 WP_032231106.1 NZ_CP060061:1979064-1979240 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T169813 NZ_CP076697:3528132-3528596 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT169813 WP_001270286.1 NZ_CP060061:1979286-1979702 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT169813 NZ_CP076697:3527797-3528132 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2X7GI71