Detailed information of TA system TA169810

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 785525..786357
• Replicon: chromosome
• Accession: NZ_CP060061
• Organism: Escherichia coli strain G6/7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: H4P50_RS03760
• Protein ID: WP_000854753.1
• Coordinates: 785525..785899 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H4P50_RS03765
• Protein ID: WP_032163902.1
• Coordinates: 785989..786357 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P50_RS03725	780592..781191	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
H4P50_RS03730	781194..782171	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
H4P50_RS03735	782168..783346	+	1179	WP_000094991.1	type II secretion system protein GspL	-
H4P50_RS03740	783348..783884	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
H4P50_RS03745	784165..784734	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
H4P50_RS03750	784831..785028	-	198	WP_000839281.1	DUF957 domain-containing protein	-
H4P50_RS03755	785040..785528	-	489	WP_000777547.1	hypothetical protein	-
H4P50_RS03760	785525..785899	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
H4P50_RS03765	785989..786357	-	369	WP_032163902.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H4P50_RS03770	786431..786652	-	222	WP_000692329.1	DUF987 domain-containing protein	-
H4P50_RS03775	786715..787191	-	477	WP_001186726.1	RadC family protein	-
H4P50_RS03780	787207..787692	-	486	WP_000849565.1	antirestriction protein	-
H4P50_RS03785	787747..788565	-	819	WP_001234620.1	DUF945 domain-containing protein	-
H4P50_RS03790	788665..788898	-	234	WP_001119729.1	DUF905 family protein	-
H4P50_RS03795	788977..789432	-	456	WP_047644727.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T169810 WP_000854753.1 NZ_CP060061:c785899-785525 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T169810 NZ_CP076697:3307056-3307433 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13501.35 Da
• Isoelectric Point: 6.6290

>AT169810 WP_032163902.1 NZ_CP060061:c786357-785989 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT169810 NZ_CP076697:3306599-3306979 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0