Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 36204..36811 Replicon plasmid unnamed1
Accession NZ_CP060058
Organism Escherichia coli strain G8

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag H4P51_RS26160 Protein ID WP_000673985.1
Coordinates 36204..36590 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag H4P51_RS26165 Protein ID WP_000493532.1
Coordinates 36587..36811 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P51_RS26140 32303..33199 + 897 Protein_39 IS3-like element IS600 family transposase -
H4P51_RS26145 33250..34677 - 1428 Protein_40 DUF4158 domain-containing protein -
H4P51_RS26150 34690..35160 + 471 WP_137493084.1 recombinase family protein -
H4P51_RS26155 35329..35607 + 279 WP_032270727.1 hypothetical protein -
H4P51_RS26160 36204..36590 - 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H4P51_RS26165 36587..36811 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H4P51_RS26170 37292..37558 + 267 WP_012605122.1 hypothetical protein -
H4P51_RS26175 37652..38647 - 996 WP_052119711.1 Gfo/Idh/MocA family oxidoreductase -
H4P51_RS26180 38658..39545 - 888 WP_016232711.1 DMT family transporter -
H4P51_RS26185 39833..40069 - 237 WP_000887751.1 hypothetical protein -
H4P51_RS26190 40553..40732 + 180 WP_000347021.1 hypothetical protein -
H4P51_RS26195 40848..41186 - 339 WP_016232712.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..99817 99817


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T169806 WP_000673985.1 NZ_CP060058:c36590-36204 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T169806 NZ_CP076697:2225727-2226101 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT169806 WP_000493532.1 NZ_CP060058:c36811-36587 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT169806 NZ_CP076697:2225270-2225638 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References