Detailed information of TA system TA169796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2614480..2615006
• Replicon: chromosome
• Accession: NZ_CP060057
• Organism: Escherichia coli strain G8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: H4P51_RS12785
• Protein ID: WP_000323025.1
• Coordinates: 2614719..2615006 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: H4P51_RS12780
• Protein ID: WP_000534858.1
• Coordinates: 2614480..2614719 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P51_RS12760	2609842..2610198	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
H4P51_RS12765	2611865..2613178	+	1314	WP_047644851.1	ISNCY family transposase	-
H4P51_RS12770	2613615..2613947	-	333	WP_001301033.1	protein FlxA	-
H4P51_RS12775	2614150..2614455	-	306	WP_001326990.1	hypothetical protein	-
H4P51_RS12780	2614480..2614719	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
H4P51_RS12785	2614719..2615006	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
H4P51_RS12790	2615078..2615233	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
H4P51_RS12795	2615450..2615701	+	252	WP_000980994.1	hypothetical protein	-
H4P51_RS12800	2615768..2616046	+	279	WP_012304870.1	hypothetical protein	-
H4P51_RS12805	2616048..2617097	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
H4P51_RS12810	2617111..2617863	+	753	WP_001047135.1	antitermination protein	-
H4P51_RS12815	2618141..2618230	-	90	WP_120795389.1	hypothetical protein	-
H4P51_RS12820	2618285..2618497	-	213	WP_000087756.1	cold shock-like protein CspF	-
H4P51_RS12825	2618798..2619013	+	216	WP_000066484.1	cold shock-like protein CspB	-
H4P51_RS12830	2619767..2619982	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2504403..2668333	163930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T169796 WP_000323025.1 NZ_CP060057:2614719-2615006 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T169796 NZ_CP076694:100509-100889 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT169796 WP_000534858.1 NZ_CP060057:2614480-2614719 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT169796 NZ_CP076694:100288-100509 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8