Detailed information of TA system TA169782

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 784683..785515
• Replicon: chromosome
• Accession: NZ_CP060057
• Organism: Escherichia coli strain G8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: H4P51_RS03750
• Protein ID: WP_000854753.1
• Coordinates: 784683..785057 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H4P51_RS03755
• Protein ID: WP_032163902.1
• Coordinates: 785147..785515 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P51_RS03715	779750..780349	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
H4P51_RS03720	780352..781329	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
H4P51_RS03725	781326..782504	+	1179	WP_000094991.1	type II secretion system protein GspL	-
H4P51_RS03730	782506..783042	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
H4P51_RS03735	783323..783892	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
H4P51_RS03740	783989..784186	-	198	WP_000839281.1	DUF957 domain-containing protein	-
H4P51_RS03745	784198..784686	-	489	WP_000777547.1	hypothetical protein	-
H4P51_RS03750	784683..785057	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
H4P51_RS03755	785147..785515	-	369	WP_032163902.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H4P51_RS03760	785589..785810	-	222	WP_000692329.1	DUF987 domain-containing protein	-
H4P51_RS03765	785873..786349	-	477	WP_001186726.1	RadC family protein	-
H4P51_RS03770	786365..786850	-	486	WP_000849565.1	antirestriction protein	-
H4P51_RS03775	786905..787723	-	819	WP_001234620.1	DUF945 domain-containing protein	-
H4P51_RS03780	787823..788056	-	234	WP_001119729.1	DUF905 family protein	-
H4P51_RS03785	788135..788590	-	456	WP_047644727.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T169782 WP_000854753.1 NZ_CP060057:c785057-784683 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T169782 NZ_CP076693:3379508-3379885 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13501.35 Da
• Isoelectric Point: 6.6290

>AT169782 WP_032163902.1 NZ_CP060057:c785515-785147 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT169782 NZ_CP076693:3379051-3379419 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0