Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 53231..54141 Replicon plasmid unnamed1
Accession NZ_CP060050
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag E4W47_RS26390 Protein ID WP_004144067.1
Coordinates 53671..54141 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag E4W47_RS26385 Protein ID WP_023287184.1
Coordinates 53231..53674 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS26370 48529..49710 + 1182 WP_023287181.1 hypothetical protein -
E4W47_RS26375 49707..51935 + 2229 WP_023287182.1 Mov34/MPN/PAD-1 family protein -
E4W47_RS26380 51982..53130 + 1149 Protein_53 translesion error-prone DNA polymerase V subunit UmuC -
E4W47_RS26385 53231..53674 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
E4W47_RS26390 53671..54141 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
E4W47_RS26395 54228..55196 + 969 WP_000654811.1 IS5 family transposase -
E4W47_RS26400 55316..55576 - 261 WP_017901409.1 hypothetical protein -
E4W47_RS26405 56150..56923 - 774 Protein_58 transposase -
E4W47_RS26410 56921..57109 - 189 Protein_59 IS481 family transposase -
E4W47_RS26415 57100..58068 + 969 WP_167876146.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA2 / dfrA12 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / catA2 - 1..199783 199783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T169775 WP_004144067.1 NZ_CP060050:53671-54141 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T169775 NZ_CP076693:c1385442-1385335 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT169775 WP_023287184.1 NZ_CP060050:53231-53674 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT169775 NZ_CP076693:1385490-1385556 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References