Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5278573..5279198 Replicon chromosome
Accession NZ_CP060049
Organism Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag E4W47_RS25750 Protein ID WP_004187928.1
Coordinates 5278573..5278956 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag E4W47_RS25755 Protein ID WP_004150355.1
Coordinates 5278956..5279198 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4W47_RS25735 5275939..5276841 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
E4W47_RS25740 5276838..5277473 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
E4W47_RS25745 5277470..5278399 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
E4W47_RS25750 5278573..5278956 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
E4W47_RS25755 5278956..5279198 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
E4W47_RS25760 5279392..5280309 + 918 WP_004187929.1 alpha/beta hydrolase -
E4W47_RS25765 5280323..5281264 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
E4W47_RS25770 5281309..5281746 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
E4W47_RS25775 5281743..5282603 - 861 WP_004146232.1 virulence factor BrkB family protein -
E4W47_RS25780 5282597..5283196 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T169774 WP_004187928.1 NZ_CP060049:c5278956-5278573 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T169774 NZ_CP076693:535734-536036 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT169774 WP_004150355.1 NZ_CP060049:c5279198-5278956 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT169774 NZ_CP076693:536072-536413 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References