Detailed information of TA system TA169773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4940041..4941722
• Replicon: chromosome
• Accession: NZ_CP060049
• Organism: Klebsiella pneumoniae strain NICU_2_P7

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A486LFH0
• Locus tag: E4W47_RS24175
• Protein ID: WP_023328808.1
• Coordinates: 4940041..4940958 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A486Q3T7
• Locus tag: E4W47_RS24180
• Protein ID: WP_023328807.1
• Coordinates: 4940964..4941722 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4W47_RS24155	4935347..4936867	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
E4W47_RS24160	4936864..4937862	+	999	WP_031592479.1	ribose ABC transporter permease	-
E4W47_RS24165	4937893..4938834	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
E4W47_RS24170	4938909..4939916	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
E4W47_RS24175	4940041..4940958	-	918	WP_023328808.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
E4W47_RS24180	4940964..4941722	-	759	WP_023328807.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
E4W47_RS24185	4941808..4942536	-	729	WP_002885152.1	response regulator transcription factor	-
E4W47_RS24190	4942809..4944956	+	2148	WP_077598921.1	formate dehydrogenase subunit alpha	-
E4W47_RS24195	4945186..4945872	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34660.13 Da
• Isoelectric Point: 5.4707

>T169773 WP_023328808.1 NZ_CP060049:c4940958-4940041 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLVEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T169773 NZ_CP076693:c506780-506562 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28582.94 Da
• Isoelectric Point: 10.0870

>AT169773 WP_023328807.1 NZ_CP060049:c4941722-4940964 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTFSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT169773 NZ_CP076693:c507180-506806 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A486LFH0

Antitoxin

• AlphaFold DB: A0A486Q3T7